Expression and clinical significance of hsa-miRNA-200c in patients with liver metastasis of epithelial ovarian cancer
- Author:
Hai-yan YE
1
;
Jia-wen XU
1
;
Le CHEN
1
;
Ke-li YOU
1
;
Wu-mei LIN
1
;
Jian-guo CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: epithelial ovarian cancer; hsa-miRNA-200c; Real-time Quantitative PCR
- From: Chinese Journal of Practical Gynecology and Obstetrics 2019;35(05):574-578
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the expressions of hsa-miRNA-200 c and its relationship with metastasis of EOC.METHODS: The expression of hsa-miRNA-200 c was detected by Stem-loop Real-time Quantitative PCR(TaqMan probe method)in 73 cases of EOC,30 cases of benign ovarian epithelial tumors and 30 cases of normal ovarian tissues,which were collected in gynecological operations from Guangdong General Hospital from October 2010 to May 2011.Meantime,the clinical pathologic features data were analyzed.The assessment of the correlation between hsa-miRNA-200 c and clinicopathological features,and the hierarchical analysis of hsa-miRNA-200 c level in 73 cases of ovarian epithelial cancer was further undertaken(Among 73 cases,13 patients suffered liver metastasis and 60 patients had non-liver metastasis).Overexpression or knockdown of hsa-miRNA-200 c,ovarian cancer cell invasion and migration abilitywas detected.RESULTS: The expression of miRNA-200 c in the EOC tissues was 382.18±15.22,which was significantly higher than that in the benign ovarian epithelial tumors(35.61 ± 1.42)and normal ovarian tissues(4.43 ±2.23)(P<0.01).There was no statistically significant difference between the latter two groups(P>0.05).The expressions of miRNA-200 c were low in EOC with late clin-ical FIGO stage(670.91±16.88 vs. 129.52±33.3,P<0.035),lymphatic metastasis(529.54±75.24 vs.175.85±49.80,P<0.004)and distant metastasis of cancer,and significantly low in hepatic metastases(377±15.31 vs. 28.22±9.14,P<0.009),but were high in ovarian endometrioid adenocarcinoma(serous carcinoma 353.89±12.51 vs.mucinous 267.93±11.43 vs.endometrioid adenocarcinoma 802.41±31.53,P<0.05);however the expressions were not correlated with pathological types or histological differentiation(P>0.05).The transwell cabinet invasion experiment showed the expression of miRNA-200 c was negatively correlated with the invasion capability of ovarian cancer cells.CONCLUSION: MiRNA-200 c is likely to play a double regulation role in the development of EOC,whose low-expression has been associated with late EOC,lymph node metastasis,liver metastasis,and poor prognosis.
