Screening and quantification of 16 β-lactam antibiotics in pork by UPLC-Q-Orbitrap
10.19485/j.cnki.issn2096-5087.2019.11.005
- VernacularTitle:猪肉中16种β-内酰胺类抗生素UPLC-Q-Orbitrap检测方法研究
- Author:
Xiaodong PAN
1
;
Baifen HUANG
;
Jingshun ZHANG
;
Zengxuan CAI
Author Information
1. Zhejiang Provincial Center for Disease Control and Prevention
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography，Quadrupole/electrostatic field orbit trap mass spectrometry，Pork，β-lactam antibiotics，Quantitative analysis
- From:
Journal of Preventive Medicine
2019;31(11):1100-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen and quantify 16 kinds of β-lactam antibiotics in pork by high performance liquid chromatography-quadrupole/electrostatic field orbit trap mass spectrometry（UPLC-Q-Orbitrap）.
Methods:The pork samples were extracted by ultrasound with acetonitrile，then the supernatant was centrifuged and purified by HLB solid phase extraction column. The analytes were separated by Waters HSS T3 column（100 mm×2.1 mm，1.8 μm）with gradient elution. Mass spectrometry adopted positive ion scanning and targeted SIM/dd-MS2 monitoring mode to complete the separation of analytes in samples and mass spectrometry analysis within 10 minutes. The chromatographic retention time and fragments in mass spectrometry were compared with prepared standards to determine whether the samples contained the antibiotics tested，then the positive samples were quantified.
Results:The 16 kinds of β-lactam antibiotics had good linear relationship in the range of 5-400 ng/mL（all the correlation coefficients >0.99）. The detection limits ranged from 0.08 μg/kg to 0.41 μg/kg，recovery rate ranged from 85.5% to 116.7%，and relative standard deviation（RSD）ranged from 3.6% to 12.8%. One of twenty pork samples detected was found penicillin G（28 μg/kg）and ampicillin（18.5 μg/kg）.
Conclusion:UPLC-Q-Orbitrap has high resolution and can reduce matrix interference to improve the accuracy. This method is simple，fast and efficient，thus can be used to screen and quantify β-lactam antibiotics in pork.
- Full text:猪肉中16种β-内酰胺类抗生素UPLC-Q-Orbitrap检测方法研究.pdf
