In vitro pro ⁃ angiogenic activity of Echinococcus granulosus hydatid cysts from experimentally infected mice
10.16250/j.32.1374.2017052
- VernacularTitle:小鼠细粒棘球蚴体外促血管生成作用研究
- Author:
Jianhai YIN
;
Yujuan SHEN
;
Aiping YU
;
Jianping CAO
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus；Angiogenesis；Matrix metalloproteinase⁃9（MMP⁃9）；High mobility group box B1 （HMGB1）；In vitro
- From:
Chinese Journal of Schistosomiasis Control
2017;29(3):320-323,333
- CountryChina
- Language:Chinese
-
Abstract:
To preliminarily study the pro⁃angiogenic activity of Echinococcus granulosus hydatid cysts against hu⁃
man umbilical vein endothelial cells in vitro and the transcriptional level of potential pro⁃angiogenic factors. Methods The hydatid cysts and protoscolex derived from experimentally infected mice were collected and cultured in vitro，then the human umbilical vein endothelial cells were stimulated by the supernatant and cyst fluid respectively，and the angiogenesis was observed and analyzed through a microscope and the angiogenesis mode of the software NIH Image J. Meanwhile，the mouse homologous proteins of matrix metalloproteinase⁃9（MMP⁃9）and high mobility group box B1（HMGB1）were identified in E. granulosus genome through sequence alignment，and their transcriptional levels in the cyst wall and protoscolex were analyzed. Results The culture supernatant of hydatid cysts significantly promoted human umbilical vein endothelial cells into tubes（F = 73.03，P < 0.001），the transcriptions of MMP⁃9 and HMGB1 were detected in the cyst wall and protoscolex，and the transcriptional level of MMP⁃9 was higher in protoscolex（t = -11.65，P < 0.001），while the level of HMGB1 was higher in hydatid cysts（t = 6.43，P = 0.003）. Conclusion Some parasite⁃derived pro⁃angiogenic molecules may exist in the supernatant of E. granulosus hydatid
cysts，while further researches are required into their exact mechanisms.
- Full text:尹建海.pdf
