Effects of Serum and its components on the biofim formation of Pseudomonas aeruginosa
10.13602/j.cnki.jcls.2019.01.03
- VernacularTitle:血清及其成分对铜绿假单胞菌生物膜形成的影响
- Author:
Yang Xia Wang
1
,
2
;
Ting Sun
1
,
2
;
Jun Fen Ma
1
,
3
;
Peng Fei She
4
;
Xiao Han Ma
1
,
2
;
Fan Wu
1
,
2
;
Jie Dan Ping
1
,
2
;
Liang Ming
1
,
2
Author Information
1. Department of Laboratory, The First Affiliated Hospital of Zhengzhou University
2. Henan Key Laboratory of Laboratory Medicine
3. Henan Key Laboratory
4. Department of Laboratory, The Third Xiangya Hospital of Central South University
- Publication Type:Journal Article
- Keywords:
horse serum;
human serum;
albumin;
transferrin;
Pseudomonas aeruginosa;
biofilm;
confocal laser scanning microscope
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of serum and its components on biofilm formation of Pseudomonas aeruginosa.
Methods:96 well microplates combined with crystal violet staining was used to detect the effects of serum, albumin and transferrin on biofilm formation of Pseudomonas aeruginosa. And confocal laser scanning microscope was used to observe the morphology of the biofilm.
Results:The biofilm of PAO1 was significantly enhanced from 2.26±0.42 to 3.42±0.08（t=4.71, p<0.01）with horse serum and but reduced to 0.807±0.10（t=4.71,p<0.01） by human serum; And the total biofilm biomass was significantly increased and clump-changed with horse serum, but decreased and scattered in distribution by human serum. Besides, horse serum could also enhance the biofilm formation of part of the clinical isolates of Pseudomonas aeruginosa, however, human serum could inhibit the biofilm formation of all of the clinical isolates. And 2.5g/L albumin could significantly enhance the biofilm of PAO1 from 1.96±0.22 to 2.54±0.18（t=3.55，p<0.05）, but 5 g/L could reduce the biofilm of PAO1 from 1.85±0.36 to 0.84±0.24（t=4.03，p<0.05）.
Conclusion:Horse serum and albumin could significantly promote the biofilm formation of Pseudomonas aeruginosa, but human serum and transferrin could decrease its biofilm formation.
- Full text:20180867-王央霞-终审稿.docx
