Application of DNA barcoding to identification of rodents in Zhejiang Province
10.19485/j.cnki.issn2096-5087.2019.05.002
- VernacularTitle:DNA条形码技术鉴定浙江省常见鼠形动物研究
- Author:
Juan HOU
1
;
Tianqi LI
;
Chunxi DING
;
Qinmei LIU
;
Rong ZHANG
;
Jian HUANG
;
Jinna WANG
;
Yuyan WU
;
Zhenyu GONG
Author Information
1. Zhejiang Provincial Center for Disease Control and Prevention
- Publication Type:Journal Article
- Keywords:
Rodents，DNA barcoding，COI gene，Identification
- From:
Journal of Preventive Medicine
2019;31(5):437-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To apply DNA barcoding to identifying the rodents in Zhejiang Province.
Methods :Rodents were captured from Jiashan,Longyou,Yunhe and Ninghai counties in Zhejiang Province. The DNA was extracted from ears of rodent samples,and was amplified and sequenced with mitochondrial cytochrome C oxidase subunit I（COI）genes. The obtained sequences were compared with the related sequences in GenBank,and neighbour-joining evolutionary tree was constructed. Then the results by DNA barcoding and by morphological identification were compared.
Results :A total of 22 COI gene samples were amplified. The evolutionary tree constructed by 18 samples was consistent with the morphological identification results and 4 samples were different：Suncus murinus should be Crocidura lasiura,infant rats of Rattus losea and Rattus tanezumi was re-identified as Rattus rattus,infant rats of Microtus fortis（sample number：NH-1）needs further identification.
Conclusion :DNA barcoding can effectively correct the errors of morphological identification,thus combining the two methods could improve the accuracy of rodent identification.
- Full text:DNA条形码技术鉴定浙江省常见鼠形动物研究.pdf
