The host investigation and virus isolation of hantavirus in Tiantai County
10.19485/j.cnki.issn2096-5087.2019.05.001
- VernacularTitle:天台县汉坦病毒宿主动物调查及病毒分离结果
- Author:
Pingping YAO
1
;
Fang XU
;
HanPing ZHU
;
Zhangnyu YANG
;
Yisheng SUN
;
Hangjing LU
;
Chen CHEN
;
Weilong PANG
;
Yun ZHANG
;
Haiqing XIANG
Author Information
1. Key Lab. of Vaccine,Prevention and Control of Infectious Disease of Zhejiang Province,Zhejiang Provincial Center for Disease Control and Prevention
- Publication Type:Journal Article
- Keywords:
Hantavirus，Hemorrhagic fever with renal syndrome，Seoul type，Rat density
- From:
Journal of Preventive Medicine
2019;31(5):433-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To learn the population,virus status and viral types of hantavirus（HV）hosts in Tiantai County of Zhejiang Province from 2011 to 2018,and to provide evidence for hemorrhagic fever with renal syndrome（HFRS）control.
Methods: Rodents in Tiantai County were captured by night trapping method. After the species and age of rodents were identified,the composition of rodent species,dominant species and density of rodents were analyzed. The lungs and blood of rodents were sampled to detect the antigen and antibody of HV by immunofluorescence method. The HV antigen-positive lung samples were detected by RT-PCR with specific primers of HV S fragment,then HV was isolated and identified by inoculating Vero-E6 cells.
Results: The average rodent density in Tiantai County from 2011 to 2018 was 4.44%. The rodent density in the field and residential areas were 4.94% and 2.23%,respectively. Ten species of rodents were identified,with Apodemus agrarius dominant in the field and Rattus norvegicus in the residential areas. Sixty-seven lung samples were HV antigen positive（4.13%）,one from Rattus norvegicus and the other sixty-six from Apodemus agrarius. Seventy-nine blood samples were HV antibody positive（4.86%）,all from Apodemus agrarius. Thirty-four HV antigen-positive lung samples were positive（50.75%）after RT-PCR amplification. Twenty-two strains of virus were isolated and all of them were from Apodemus agrarius,including twenty-one strains of Hantaan type（HTN）and one strain of Seoul type（SEO）.
Conclusion :In Tiantai County,Apodemus agrarius is the main source of HFRS infection;the main epidemic type of HV is HTN and SEO is first found in Apodemus agrarius.
- Full text:天台县汉坦病毒宿主动物调查及病毒分离结果.pdf
