Screen of differentially expressed genes in liver cancer xenograft tissues after CDC25Agene silence
10.3872/j.issn.1007-385x.2020.02.004 004
- VernacularTitle:沉默CDC25A基因后肝癌裸鼠移植瘤组织中差异表达基因的筛选
- Author:
CHEN Si
1
;
TANG Yanping
1
;
LI Kezhi
1
;
YANG Chun
1
;
HUANG Xiaoqing
1
;
CHEN Xiujuan
1
;
CAO Ji
1
Author Information
1. Department of Experimental Research, Cancer HospitalAffiliated to Guangxi Medical University
- Publication Type:Journal Article
- Keywords:
liver cancer;
cell division cycle 25A(CDC25A);
human gene expression chip;
xenograft tumor;
GO analysis;
KEGG analysis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(2):123-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the genes that may be regulated by cell division cycle 25A (CDC25A) with gene chip technology, and to elucidate and verify that CDC25A has a regulatory effect on the expression of liver cancer related genes. Methods: CDC25A expression in human liver cancer HepG2 cells was silenced by siRNA interference technology and a nude mouse xenograft model of liver cancer was successfully constructed in our previous research. Affymetrix human gene expression profiling microarray was used to further screen differentially expressed genes (DEGs) after silencing CDC25A in liver cancer xenografts, and GO analysis and KEGG analysis were performed. Some of the DEGs were verified by qPCR. Results: The chip screened 188 DEGs in liver cancer xenograft tissues after CDC25A silence, including 78 up-regulated genes and 110 down-regulated genes. These DEGs mainly involved in cell proliferation, apoptosis, protein complex binding, extracellular space, etc., and associated with the changes in pathways such as focal adhesions and extracellular matrix (ECM) receptor interactions. qPCR showed that the expression of HIPK2 mRNA was up-regulated and the mRNA expressions of (microfibrillar-associated protein 5(MFAP5) and cyclin D1 (CCND1) were down-regulated, which were consistent with the results of microarray detection. Conclusion: Using human gene expression profiling chip, the DEGs in liver cancer xenograft tissues in nude mice after silencing CDC25Awere successfully screened, providing effective clues for exploring the effect of CDC25Aon the growth of liver cancer.
- Full text:20200204.pdf
