Study on the efficacy evaluation of real-time fluorescence assay of RNA isothermal amplification in the treatment of patients with positive pulmonary tuberculosis
- VernacularTitle:RNA恒温扩增实时荧光检测技术对复治菌阳 肺结核患者疗效评价的研究
- Author:
Shu-jun GENG
1
Author Information
1. Department of Tuberculosis Medicine,Hebei Chest Hospital, Hebei 050041, China
- Publication Type:Journal Article
- Keywords:
nucleic acid amplification technology;
tuberculosis, lung;
mycobacterium tuberculosis;
drug efficacy
- From:
Tianjin Medical Journal
2018;46(9):956-959
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of real-time fluorescence detection technique of RNA (SAT) constantamplification
in monitoring the effect of chemotherapy on patients with sputum positive pulmonary tuberculosis. Methods
Sixty-two patients were selected, who were clinically diagnosed as the first-time retreatment for sputum smear-positive
pulmonary tuberculosis and were hospitalized in our department from June 2015 to December 2016. After two-month
standard anti-tuberculosis treatment, sputum samples were detected by Peng’s vessel acid-fast staining, BACTEC MGIT-
960 culture and strain identification, SAT detection. The BACTEC MGIT-960 culture and strain identification were used as
gold standards, the value of SAT during the monitoring the therapeutic effect of the anti-tuberculosis drugs was assessed.
Results After the treatment, 49 cases out of 62 showed positive results in mycobacterium tuberculosis culture test, among
them 42 patients were diagnosed as human type mycobacterium tuberculosis, 7 patients were diagnosed as nontuberculous
mycobacteria infection, and 13 cases showed negative results in mycobacterium tuberculosis culture. Thirty-two cases
showed positive results in sputum Peng’s vessel acid-fast staining test, and 30 cases showed negative results. Forty-one
cases showed positive results in SAT test and 21 cases showed negative results in SAT. SAT results were well concordant
with sputum culture results (Kappa value=0.964), and the sensitivity, the specificity, the positive predictive value and the
negative predictive value of SAT were 97.62%, 100%, 100% and 95.24% respectively. Peng’s vessel acid-fast staining
results were badly concordant with MGIT-960 culture results (Kappa value=0.086), and the sensitivity, the specificity, the
positive predictive value and the negative predictive value of Peng’s vessel acid-fast staining were 54.76%, 55.00%,
71.88% and 36.67% respectively. Conclusion SAT results can be used as good indices during the monitoring therapeutic
effects of drugs used for the first-time retreatment in patients with sputum smear-positive pulmonary tuberculosis, which is
worth promoting.
