Measurement of Rho-kinase and CD4+CD25+ regulatory T cells in the peripheral blood in asthmatic patients.
10.11817/j.issn.1672-7347.2014.06.006
- Author:
Xiaojie DENG
1
;
Hongzhi ZHU
;
Shangjie WU
Author Information
1. Department of Respiratory Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Asthma;
blood;
Case-Control Studies;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Forced Expiratory Volume;
Humans;
T-Lymphocytes, Regulatory;
cytology;
Vital Capacity;
rho-Associated Kinases;
blood
- From:
Journal of Central South University(Medical Sciences)
2014;39(6):577-581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the levels of Rho-kinase and CD4+CD25+ regulatory T cells in patients with asthma, and the relationship between Rho-kinase and CD4+CD25+ regulatory T cells.
METHODS:We included 16 patients with moderate to severe asthma in the research group and 14 healthy people as the control group. The levels of Rho-kinase in the 2 groups were measured by ELISA. The level of CD4+CD25+ regulatory T cells in the 2 groups was measured by flow cytometry. The pulmonary function was measured by spirometer.
RESULTS:The level of Rho-kinase in the research group was higher than that in the healthy controls (P<0.05). The level of CD4+CD25+ regulatory T cells in the healthy controls was higher than that of the research group (P<0.05). There was no correlation between the level of Rho-kinase in the peripheral blood of the 2 groups and forced expiratiory volume at the first second/ forced vital capacity (FEV1%) (r=-0.491, P>0.05). The level of CD4+CD25+ regulatory T cells in the peripheral blood of the 2 groups showed a positive correlation with FEV1% (r=0.380, P=0.038). There was no correlation between the level of Rho-kinase and the level of CD4+CD25+ regulatory T cells in the peripheral blood of the 2 groups (r=-0.438, P>0.05).
CONCLUSION:Rho-kinase and CD4+CD25+ regulatory T cells may play a key role in the pathogenesis of asthma.
