Effect of verapamil and nitroglycerin on transplanted lung function in canines.
10.3969/j.issn.1672-7347.2014.08.008
- Author:
Zhibin JIANG
1
;
Ping HU
;
Jianxin LIU
;
Dianjun WANG
;
Longyu JIN
;
Chao HONG
Author Information
1. Department of Cardiothoracic Surgery, Third Xiangya Hospital, Central South University, Changsha 410013,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Gas Analysis;
Dogs;
Hemodynamics;
Lung;
Lung Transplantation;
Nitroglycerin;
pharmacology;
Organ Preservation;
Protective Agents;
Reperfusion Injury;
Verapamil;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2014;39(8):802-806
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protective effect of combined administration of verapamil and nitroglycerin on the function of canine transplanted lungs.
METHODS:Twenty orthotopic left lung transplantations were performed in 40 canines, which were randomly divided into 4 groups. In group I (control), the donor lungs were perfused and preserved with LPD solution, while group II with LPD solution plus verapamil 0.1 g/L, group III with LPD solution plus nitroglycerin 0.1g/L, and group IV with LPD solution plus verapamil 0.1 g/L and nitroglycerin 0.1 g/L. Hemodynamics and graft gas exchange were assessed 0, 2 and 4 h after the operation. The lung grafts were harvested to measure the wet/dry weight ratio, malondialdehyde (MDA) contents and superoxide dismutase (SOD) activity.
RESULTS:Compared with group I, II and III, the mean pulmonary artery pressure (MPAP), pulmonary vascular resistance index (PVRI), partial pressure of oxygen in arterial blood (PaO₂), dynamic compliance (Cdyn) and alveolar-arterial oxygen tension volume [P(A- a)O₂] in group IV were improved significantly (P<0.05). No significant difference in the partial pressure of carbondioxide (PaCO₂) and peak inspiratory pressure (PIP) was observed in the 4 groups (P>0.05). In group IV, the wet/dry weight ratio and MDA contents were lower than those in the other 3 groups, and the SOD activity was significantly higher than that of the other 3 groups (P<0.05).
CONCLUSION:Verapamil and nitroglycerin in LPD solution can protect the respiratory function of canine lung grafts by attenuating pulmonary edema and oxidative stress.
