Peroxiredoxin I in sperm and reactive oxygen species in seminal plasma in patients with idiopathic asthenozoospermia.
10.3969/j.issn.1672-7347.2014.08.015
- Author:
Haiyan WANG
1
;
Nenghui LIU
;
Hong ZENG
Author Information
1. Reproductive Medical Center, Department of Obstetrics and Gynaecology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Asthenozoospermia;
Case-Control Studies;
Humans;
Male;
Peroxiredoxins;
metabolism;
Reactive Oxygen Species;
chemistry;
Semen;
chemistry;
Sperm Motility;
Spermatozoa;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2014;39(8):842-848
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between peroxiredoxin I expression and seminal reactive oxygen species (ROS) in patients with idiopathic asthenozoospermia.
METHODS:Twenty-six infertile male patients were selected from the Reproductive Medical Center, Xiangya Hospital, from September to December in 2012. Fresh semen was collected from an experimental group (26 idiopathic asthenozoospermia patients) and a control group (15 men with fertility history and normal semen). Luminol chemiluminescence method was applied to detect the seminal ROS level. Western blot was used to detect the peroxiredoxin I expression.
RESULTS:1)The seminal ROS level in the experimental group was significantly increased compared with that in the control group (P<0.05), and the seminal ROS level was negatively correlated with mobility of the sperm (r=-0.777, P<0.01). 2) Compared with the control group, the peroxiredoxin I expression was significantly downregulated in the experimental group (P<0.05). The content of sperm peroxiredoxin I in the 2 groups was negatively correlated with the seminal ROS level (r=-0.474, P<0.01). 3) The content of peroxiredoxin I had a positive correlation with human sperm motility(r=0.779, P<0.01).
CONCLUSION:The decline of peroxiredoxin I expression may be one of the crucial factors that leads to idiopathic asthenozoospermia. High level of ROS may be one of the main reasons for sperm vitality decline in patients with idiopathic asthenozoospermia.
