Effect of Feridex-GFP double-labeled BMSC transplant on the damaged liver under the condition of constant magnetic field.
10.11817/j.issn.1672-7347.2014.10.004
- Author:
Shaojun LIU
1
;
Xiaoyan WANG
;
Zhiming XIAO
;
Anliu TANG
;
Wuliang TANG
;
Shourong SHEN
Author Information
1. Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Dextrans;
Green Fluorescent Proteins;
Liver Diseases;
therapy;
Magnetic Fields;
Magnetite Nanoparticles;
Mesenchymal Stem Cell Transplantation;
Rats
- From:
Journal of Central South University(Medical Sciences)
2014;39(10):1008-1015
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the effect of the bone marrow mesenchymal stem cells (BMSCs) transplant through peripheral vein, portal vein and hepatic artery into liver under the condition of constant magnetic field and to analyze the therapeutic effect on liver function recovery.
METHODS:BMSCs were isolated, purified and induced to differentiate into liver-like cells, which were double labeled by Feridex-GFP. The double-labeled BMSCs were transplanted into liver through different ways including peripheral vein, portal vein and hepatic artery with or without constant magnetic field in vitro. The rats were sacrificed at the 1st, 2nd, 3rd and 4th week after the transplant. ALB, ALT, AST were tested. The liver tissue biopsy was collected. GFP-positive cells in liver were observed by fluorescence microscopy.
RESULTS:Double-labeled BMSCs could be transplanted into liver through all ways. GFP expression was found in liver in all groups at the 4th week and the liver functions were improved. Based on the long term efficacy, the liver functions recovered more rapidly in the portal vein + constant magnetic field group and the hepatic artery + constant magnetic field group.
CONCLUSION:BMSCs transplantation can reduce acute liver damage. The first choice for BMSCs transplantation was via portal vein or hepatic artery under the condition of constant magnetic field. The second choice was via peripheral vein alone or under the condition of constant magnetic field.
