Nucleolus expression in diabetic cardiomyopathy.
10.11817/j.issn.1672-7347.2014.10.012
- Author:
Li SUN
1
;
Xin HE
;
Juan WANG
;
Bimei JIANG
;
Zhongyi TONG
;
Yanjuan LIU
;
Yuanbin LI
;
Xianzhong XIAO
Author Information
1. Department of Pathophysiology, School of Basic Medical Science, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Glucose;
Diabetes Mellitus, Experimental;
metabolism;
Diabetic Cardiomyopathies;
metabolism;
Myocardium;
pathology;
Phosphoproteins;
metabolism;
RNA-Binding Proteins;
metabolism;
Rats
- From:
Journal of Central South University(Medical Sciences)
2014;39(10):1056-1060
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the nucleolus expression in the diabetic cardiomyopathy.
METHODS:The rats were divided into a control group and a type II diabetic cardiomyopathy group (model group). In the model group, rats were fed with high-fat and high-sugar food (rats were intravenously injected with 60 mg/kg chain urea with cephalosporins in the 5th and 6th weeks in mice). The level of blood glucose was determined at the end of 8th week and the level of fasting blood glucose was examined at the end of 20th week. The ratio of the heart mass and body mass was calculated, and the pathological changes in myocardial morphology were observed. The immunohistochemical method and Western blot were used to detect the expression level of myocardial nucleolin.
RESULTS:The level of fasting blood glucose was significantly increased in the diabetic model group than that in the control group (P<0.05). Rats in the model group were found hypertrophic cardic cells, with fracture, dissolusion, and disordered arrangement. Immunohistochemical staining and Western blot showed the protein levels of myocardial nucleolin in the model group were obviously higher than those in the control group (P<0.05).
CONCLUSION:Nucleolin may play a role in the pathogenesis and development of the diabetic cardiomyopathy.
