Pharmacological characteristics of glycine receptors in rat hippocampal pyramidal cells.
10.11817/j.issn.1672-7347.2014.10.001
- Author:
Kai MENG
1
;
Wen XIE
;
Li ZHANG
Author Information
1. Department of Physiology and Pathophysiology, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CA1 Region, Hippocampal;
cytology;
Glycine;
pharmacology;
Patch-Clamp Techniques;
Pyramidal Cells;
drug effects;
Rats;
Receptors, Glycine;
metabolism;
Strychnine;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2014;39(10):989-993
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate function of glycine receptors (GlyRs) at the hippocampal CA1 pyramidal cells and to characterize the pharmacological properties of these receptors at early postnatal stage.
METHODS:We used whole cell patch clamp recording to study the current response in the acutely prepared hippocampal slices from postnatal day 11-13 rats induced by glycine applied in the artificial cerebrospinal fluid.
RESULTS:Application of glycine to the pyramidal cells elicited strychnine sensitive chloride currents. EC50 for GlyRs respond to glycine was 123. 23 μmol/L and Hill coefficient was 1.24. Picrotoxin could partly blocked the currents.
CONCLUSION:Strychnine sensitive glycine receptors are functionally expressed in CA1 pyramidal neurons in rat hippocampal CA1 area at early postnatal stage, and some of GlyRs are αβ heteromeric receptors.
