Protective effect of NMDA receptor antagonist memantine on acute lung injury in mice.
10.11817/j.issn.1672-7347.2014.01.003
- Author:
Ling MA
1
,
2
;
Wei LIU
;
Dandan FENG
;
Jianzhong HAN
;
Yang LI
;
Qingmei CHENG
;
Shaojie YUE
;
Ziqiang LUO
Author Information
1. Department of Physiology, Xiangya School of Basic Medicine, Central South University, Changsha 410078
2. Yongzhou Vocational and Technical College, Yongzhou Hunan 425000, China).
- Publication Type:Journal Article
- MeSH:
Acute Lung Injury;
drug therapy;
Animals;
Bronchoalveolar Lavage Fluid;
L-Lactate Dehydrogenase;
metabolism;
Lipopolysaccharides;
Lung;
metabolism;
Male;
Malondialdehyde;
metabolism;
Memantine;
pharmacology;
Mice;
Peroxidase;
metabolism;
Receptors, N-Methyl-D-Aspartate;
antagonists & inhibitors;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2014;39(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protective effect of a non-specific NMDA receptor antagonist memantine on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.
METHODS:Healthy male mice were divided into 4 groups: a normal group, a memantine group, an ALI group and a memantine+ALI group. The ALI group was induced by intraperitoneal injection of LPS (10 mg/kg). Memantine (10 mg/kg) was injected intraperitoneally before the injection of LPS to determine the effect of blockade of NMDA receptor in the memantine+ALI group. The lung wet/dry ratio was detected. HE staining was preformed to show the morphological changes in the lung tissue. Myeloperoxidase enzyme (MPO) activity and malondialdehyde (MDA) content in the lung tissue were detected. ELISA was used to detect the tumor necrosis factor-α (TNF-α) content and lactate dehydrogenase (LDH) activity in the bronchoalveolar lavage fluid (BALF).
RESULTS:Memantine pretreatment improved the LPS-induced ALI lung tissue morphological changes, reduced their lung wet/dry ratio, the levels of TNF-α and LDH activity in BALF, and also reduced the MPO and MDA content in the lung tissue.
CONCLUSION:Blockade of NMDA receptors can ameliorate LPS-induced mice ALI.
