Influence of hepatocyte growth factor on iNOS, NO and IL-1β in the cerebrum during cerebral ischemia/reperfusion in rats.
10.11817/j.issn.1672-7347.2014.01.005
- Author:
Fang HE
1
;
Bei YE
;
Jianzhen CHEN
;
Xiaoyan SUN
;
Chang LI
Author Information
1. Department of Basic Medicine, Suzhou Health College, Suzhou Jiangsu 215009, China fanghe126@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain Ischemia;
metabolism;
Cerebrum;
metabolism;
pathology;
Down-Regulation;
Hepatocyte Growth Factor;
pharmacology;
Interleukin-1beta;
metabolism;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type II;
metabolism;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
metabolism;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2014;39(1):23-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of hepatocyte growth factor (HGF) on inducible nitric oxide synthase (iNOS), NO and interleukin-1β (IL-1β) in the cerebrum of rats subjected to cerebral ischemia/reperfusion (I/R).
METHODS:Sprague-Dawley rats were randomly divided into 5 groups: a sham group, an I/R group,an HGF1 group, an HGF2 group, and an HGF3 group. The latter 3 groups were respectively injected 15, 30 and 60 μg/kg HGF. The focal cerebral I/R model was established by sutureoccluded method. After 1.5 h ischemia followed by 24 h reperfusion, the iNOS activity and NO content in the ischemic cerebral tissue were assessed. The expression of iNOS mRNA and IL-1β mRNA was detected. The level of iNOS protein and IL-1β content were determined. In addition, cultured cerebral cortical neurons in vitro were exposed to I/R. Then the expression of iNOS and IL-1β protein in the neurons was detected, and NO content was assessed.
RESULTS:The iNOS activity and NO content in the ischemic cerebral tissue were increased. The expression of iNOS mRNA and IL-1β mRNA was upregulated. The level of iNOS protein and IL- 1β content were increased. Administration of HGF decreased the iNOS activity and NO content, and downregulated the expression of iNOS mRNA, IL-1β mRNA, iNOS protein and IL-1β content in the ischemic cerebral tissue. HGF decreased the expression of IL-1β, iNOS protein and NO content in the cortical neurons exposed to I/R in vitro.
CONCLUSION:HGF can inhibit the expression of IL-1β and decrease the expression of iNOS and content of NO, which is probably one of the mechanisms mediating the protection of HGF against cerebral ischemia injury.
