Role of Notch-Jagged/Delta signaling pathway in arthritis rats of reduced lung function induced by adjuvant.
10.11817/j.issn.1672-7347.2014.02.001
- Author:
Lei WAN
1
;
Jian LIU
;
Chuanbing HUANG
;
Yuan WANG
;
Xiaojun ZHANG
;
Liping RUAN
;
Yali WANG
;
Wenfang YE
Author Information
1. Rheumatology Division, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Arthritis, Experimental;
metabolism;
physiopathology;
Calcium-Binding Proteins;
metabolism;
Freund's Adjuvant;
Intercellular Signaling Peptides and Proteins;
metabolism;
Intracellular Signaling Peptides and Proteins;
metabolism;
Jagged-1 Protein;
Lung;
metabolism;
pathology;
Membrane Proteins;
metabolism;
Rats;
Receptors, Notch;
metabolism;
Respiratory Insufficiency;
metabolism;
physiopathology;
Serrate-Jagged Proteins;
Signal Transduction;
Vital Capacity
- From:
Journal of Central South University(Medical Sciences)
2014;39(2):109-116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the changes of pulmonary function and Notch signaling pathway of lung tissues in adjuvant-induced arthritis rats, and to investigate the mechanism of reduced lung function.
METHODS:A total of 30 rats were randomly divided into a normal group and a model group. Rats in the model group were induced to establish the adjuvant arthritis AA model by intradermally injecting 0.1 mL Freund's complete adjuvant into the right paw. After 30 days, we observed the paw edema volume, arthritis index, pulmonary function, histomorphology, and Notch receptor/ligand of the lung tissue.
RESULTS:Compared with the normal group, the paw edema volume, arthritis index, average expiratory flow within 0.3 s (FEV0.3/FVC), and the level of Notch3, Notch4 and Jagged2 of the lung tissue in the model group was significantly increased, while maximum expiratory flow at 50% of vital capacity (FEF50), maximum expiratory flow at 75% of vital capacity (FEF75), forced expiratory flow (PEF) and the expression of Notch1 of Jagged1 and Delta1 in the lung were significantly decreased (P<0.05, P<0.01). There were significant positive correlations between FEV0.3/FVC and Notch4. FEV0.3/FVC, FEF25, FEF50 and Notch3, Delta1 were negatively correlated, respectively (P<0.05, P<0.01).
CONCLUSION:While arthritis occurs in AA rats, pulmonary function declines and significantly correlates with the expression of Notch receptor/ligand. The deposition of immune complex in the lung after the injection of CFA activates the Notch signaling pathway, and results in further decline of pulmonary function by signaling cascades.
