Expression of nucleolin in pressure overload-induced cardiac hypertrophy rats.
10.11817/j.issn.1672-7347.2014.02.003
- Author:
Simin YAN
1
;
Shuang WU
;
Li SUN
;
Bimei JIANG
;
Zizhi TU
;
Xianzhong XIAO
Author Information
1. Clinical Medical Major, Grade 2008,Xiangya School of Medicine, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Pressure;
Cardiomegaly;
metabolism;
Myocardium;
metabolism;
Phosphoproteins;
metabolism;
RNA, Messenger;
RNA-Binding Proteins;
metabolism;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2014;39(2):124-128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expression of nucleolin in cardiac hypertrophy rats induced by pressure overload.
METHODS:A total of 40 SD rats with body weight 180 g and 220 g were recruited and randomly divided into 2 groups: a transverse aortic constriction (TAC) group and a sham surgery group. Cardiac hypertrophy model was employed by transverse aortic constriction surgery. Then 2 weeks and 4 weeks after the experiment, the heart mass index (HMI), left ventricle mass index (LVMI) were measured. β-MHC mRNA in the heart tissue was detected with RT-PCR. Nucleolin in the heart, brain and kidney was respectively detected with Western blot.
RESULTS:Compared with the sham surgery group, HMI, LVMI in the TAC group increased significantly (P<0.01) 4 weeks after the surgery; the expression of β-MHC mRNA in the heart tissue increased (P<0.05) in the TAC group 4 weeks after the surgery; and the expression of nucleolin protein in the heart tissue of the TAC group was remarkably upregulated (P<0.05) 2 weeks after the surgery, with no change in the brain and kidney tissue between the 2 groups.
CONCLUSION:Expression of nucleolin protein has been upregulated in response to pressure overload, which may suggest that nucleolin plays a role in cardiac hypertrophy induced by pressure overload.
