Endothelial cells promote islet survival and function.
10.11817/j.issn.1672-7347.2014.02.004
- Author:
Xiaoming PAN
1
;
Chenguang DING
;
Wujun XUE
;
Xinshun FENG
;
Yong SONG
;
Xiaohui TIAN
Author Information
1. Nephropathy Center, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061,China drpanxiaoming@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Glucose;
analysis;
Coculture Techniques;
Diabetes Mellitus, Experimental;
Endothelial Cells;
cytology;
Graft Survival;
Insulin;
blood;
Islets of Langerhans;
cytology;
Islets of Langerhans Transplantation;
Rats
- From:
Journal of Central South University(Medical Sciences)
2014;39(2):129-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate islet graft survival and function after co-culture and co-transplantation with vascular endothelial cells (ECs) in diabetic rats.
METHODS:We isolated ECs, and assessed the viability of isolated islets in a group of standard culture and a group of co-culture with ECs. Then we put the diabetic rats in 4 groups: an islet transplantation group, an islet graft with EC transplantation group, an EC transplantation group, and a PBS control group. Blood glucose and insulin concentrations were measured daily. Cell morphology and cell markers were investigated by immunohistochemical staining and electron microscope.
RESULTS:Normal morphology was shown in more than 90% of AO/PI staining positive islets while co-cultured with ECs for 7 days. Insulin release assays showed a significantly higher simulation index co-culture except for the first day (P<0.05). There was a significant difference in concentrations of blood glucose and insulin among the 4 groups after 3 days after the transplantation (P<0.05).
CONCLUSION:EC-islet co-culture can improve the function and survival of isolated islets in vitro, and EC-islet co-transplantation can effectively prolong the islet graft survival in diabetic rats.
