Methodology of BMSCs in repairing ureteral injury in mice via renal artery transplantation.
10.11817/j.issn.1672-7347.2014.03.010
- Author:
Donghui LI
1
;
Zhiming BAI
;
Jindong LI
;
Qing CHENG
;
Ziyi GUO
;
Xiangdong DENG
;
Hui CAO
Author Information
1. Department of Urology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Hematopoietic Stem Cell Transplantation;
methods;
Hematopoietic Stem Cells;
cytology;
Mice;
Mice, Inbred BALB C;
Renal Artery;
Ureteral Obstruction;
therapy
- From:
Journal of Central South University(Medical Sciences)
2014;39(3):282-289
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on ureteral injury repair via renal artery transplantation.
METHODS:The left ureteral obstruction model was set up in 49 Balb/c mice by micro vascular clamp. The microscopic vascular clamp was taken out to lift the left ureteral obstruction after 10 days. The mice were randomly divided into an experimental group (n=25) and a control group (n=24). Balb/c mice BMSCs transfected by luciferase (Luc) were transplanted immediately through the renal artery after removing the microscopic vascular clamp from the experimental group; while mice in the control group was closed the incision after the microscopic vascular clamp was removed immediately and without BMSCs transplant. Magnetic resonance imaging (MRI) was used to scan the experimental mice. By measuring the left renal pelvis volume of the experimental mice at different time points and comparing the left ureter recanalization rate after removing left ureteral obstruction of the experimental group and the control group, we evaluated the repair effect of BMSCs on ureteral injury.
RESULTS:The volume of the left renal pelvis in experimental mice became bigger obviously after the left ureter was obstructed (P<0.01). The left renal pelvis volume of the experimental group and the control group had no statistical significance 10 days after the left ureteral obstruction was set up (P=0.693). In the experimental group, the left ureter recanalization rate was higher than that in the control group, after removing the left ureteral obstruction (P=0.012).
CONCLUSION:Transplantation through the renal artery can promote the restoration of ureteral injury in mice.
