Effect of stress on myocardial apoptosis in ischemic preconditioning in rabbit hearts.
10.3969/j.issn.1672-7347.2014.05.007
- Author:
Huashan XU
1
,
2
;
Shengxi CHEN
;
Wanjun LUO
;
Khan Mohammed FIROJ
Author Information
1. Department of Cardiovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052
2. Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Etomidate;
pharmacology;
Heart;
drug effects;
Ischemic Preconditioning;
Ischemic Preconditioning, Myocardial;
Methylprednisolone;
pharmacology;
Myocardium;
pathology;
Rabbits
- From:
Journal of Central South University(Medical Sciences)
2014;39(5):477-482
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the influence of stress on myocardial apoptosis in ischemic preconditioning group (IPC).
METHODS:Twenty-four Japanese white rabbits were randomly divided into 4 groups (n=6): an etomidate group (the Etom group) of depressed stress established by intravenous etomidate, an IPC group, an ischemic reperfusion group (the IR group) and a methylprednisolone group (the MP group). Myocardial apoptosis was examined by DNA-laddering, in situ nick-end labeling (TUNEL) and Hoechst dyeing.
RESULTS:The DNA ladder increased in the Etom group. The percentage of apoptosis by TUNEL method was 1.7%±0.2% in the IPC group, 2.3%±0.8% in the MP group, 3.8%±1.3% in the IR group and 3.0%±0.4% in the Etom group. Hoechst dying was 4.1%±0.9% in the IPC group, 3.5%±0.4% in the MP group, 6.2%±1.6% in the IR group and 7.6%±0.4% in the Etom group. There was significant difference between the IPC group and the Etom group or IR group, and also between the MP group and the IR group.
CONCLUSION:A depressed stress response impairs the inhibition on myocardial apoptosis in ischemic preconditioning. Methylprednisolone may inhibit myocardial apoptosis.
