The role of Synaptotagmin1 in mouse oocyte fertilization

VernacularTitle:Synaptotagmin1在小鼠卵母细胞体外受精过程中的作用
Author: Xiu-lan ZHU ¹
Author Information

1. Department of Reproductive Health and Infertility, Guangdong Women and Children Hospital, Guangzhou 510000, China
Publication Type:Journal Article
Keywords: fertilization; zygote; exocytosis; Synaptotagmin1
From: Tianjin Medical Journal 2018;46(9):928-931
CountryChina
Language:Chinese
Abstract: Objective To investigate the dynamics of cortical granules exocytosis (CGE), and the role of Synaptotagmin1 (Syt1) in mouse fertilization. Methods The dynamics of mouse CGE were filmed on Perkin Elmer precisely Ulta VIEW VOX confocal Imaging System. The Syt1 functions on mouse fertilization and expression in zygotes were analyzed by immunofluorescence, Western blot assy and qRT-PCR. Results The dynamic process of mouse CGE was observed after oocyte activation, and the exact pot seems to be close to the polar body. The Syt1 expression was gradually increased after fertilization. The mouse fertilization rate after Syt1 knockdown was significantly lower than that of the control group (P＜ 0.05). Conclusion The dynamic process of CGE is studied. It is found that Syt1 is involved in mouse fertilization process.