The inhibitory effect and molecular mechanism of delphindin on HER-2 positive breast cancer
- VernacularTitle:飞燕草素对HER-2阳性乳腺癌的抑制效应及 分子机制研究
- Author:
Ai-lin WU
1
Author Information
1. Chengdu Medical College, Chengdu 610000, China
- Publication Type:Journal Article
- Keywords:
receptor, epidermal growth factor;
antineoplastic agents, phytogenic;
breast neoplasms;
cell cycle;
delphinidin;
HER-2 positive breast cancer;
NF-κB signaling pathway
- From:
Tianjin Medical Journal
2018;46(9):910-915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect and molecular mechanism of delphinidin (Dp) on HER-2
positive breast cancer. Methods The HER-2 positive breast cancer cells (MDA-MB-453 and BT-474) were treated with
different concentrations of delphinidin (10，20，40，80 and 160 μmol/L) for 48 hours. The same concentration of DMSO was
used as the solvent control. CCK-8 method was used to measure the effect of Dp on cell activity, and half maximal inhibitory
concentration (IC50) was calculated to determine the concentration of subsequent experiments. The cells were treated with
different concentrations of Dp (20, 40 and 80 μmol/L) for 48 hours. HE staining was used to observe the cell morphological
changes. Flow cytometry was used to analyze the cell cycle. Cell apoptosis rate was detected by TUNEL assay. The
phosphorylation levels of NF - κB signaling pathway related proteins were determined by Western blot assay. Results
Delphinidin inhibited the proliferation of MDA-MB-453 and BT-474 in the concentration ranges of 10，20，40，80 and 160 μmol/L (IC50: 41.02 and 60.97 μmol/L). In the concentrations of 20，40 and 80 μmol/L, compared with the control group, it
was found that some cells were detached, floated and lysed, and the cell volume was decreased, the proportion of cells in G2/
M phase and the apoptosis rate were increased in DP treatment groups. Compared with the control group, the expression
levels of p-NF-κB/p65, p-IκBα, p-IKKα/β and p-PKCα were significantly decreased in the 40 and 80 μmol/L Dp
treatment groups, while the expression levels of IκBα, IKKα, IKKβ and PKCα were increased in the Dp treatment groups
(P＜0.05). Conclusion Delphinine can inhibit the proliferation of breast cancer cells by blocking the NF-κB signaling
pathway and inducing the G2/M cycle arrest and apoptosis of MDA-MB-453 and BT-474 cells.
Key words：receptor, epidermal growth factor; antineoplastic agents, phytogenic; breast neoplasms; cell cycle;
delphinidin;HER-2 positive breast cancer; NF-κB signaling pathway
