The protective effect of IGF-1 on 6-OHDA induced oxidative damage in neurons
- VernacularTitle:IGF-1对6-OHDA诱导神经元氧化损伤的保护作用
- Author:
Xiao-guang DONG
1
Author Information
1. Department of Spine Surgery, Qingdao Orthopedic Hospital, Qingdao 266100, China
- Publication Type:Journal Article
- Keywords:
insulin-like growth factor Ⅰ;
hydroxydopamines;
PC12 cells;
oxidative stress;
apoptosis;
Parkinson disease;
6-hydroxy dopamine
- From:
Tianjin Medical Journal
2018;46(9):905-910
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of insulin-like growth factor-1 (IGF-1) on 6-hydroxy
dopamine (6-OHDA) induced oxidative damage in PC-12 cells. Methods PC12 cells were treated with 6-OHDA
(concentrations of 25, 50, 100, 150 and 200 μmol/L). In order to select the optimal experimental concentration and treatment
time, the activity of PC12 cells was detected by MTT at different time points of 12 h, 24 h and 48 h after treatment. PC12
cells were divided into three groups: control group, 6-OHDA group and IGF-1+6-OHDA group. The activity of PC12 cells
was detected by MTT assay. Reactive oxygen species (ROS) level of PC12 cells was detected by immunofluorescence
staining, and apoptosis was detected by Hoechst33342 / PI double staining method. Results With the increased
concentration and the prolongation of the action time of 6-OHDA, the activity of PC12 cells decreased gradually. The
concentration of 150 μmol/L and action time of 24 h of 6-OHDA were selected as the optimal experimental concentration
and observation time for this study. Compared with 6-OHDA group, the activity of PC12 cells increased, the expression level
of ROS and the apoptosis decreased in IGF-1+6-OHDA group. Conclusion IGF-1 pretreatment can reduce 6-OHDA
induced oxidative damage and apoptosis of PC12 cells, also can increase cell activity, which can provide a potential strategy
for the prevention and treatment of Parkinson’s disease.
