Knockdown of circ_0001273 inhibits the proliferation, migration and invasion of oral squamous cell carcinoma cells
10.12016/j.issn.2096-1456.2020.03.003
- Author:
ZENG Fantao
1
;
YU Dongsheng
2
Author Information
1. Department of Stomatology, the Second Affiliated Hospital of Guangzhou Medical University
2. Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology
- Publication Type:Journal Article
- Keywords:
oral squamous cell carcinoma cells;
squamous cell;
carcinoma;
circRNA;
circ_0001273;
OSCC cell lines UM1;
OSCC cell lines CAL27;
proliferation;
migration;
invasion
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(3):153-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of circ_0001273 on the proliferation, migration and invasion of oral squamous cell carcinoma（OSCC） cells and to provide a relevant research basis for the use of targeted therapy in OSCC.
Methods:Data from twelve patients with a clinical diagnosis of OSCC were collected from tumor specimens and adjacent tissues. qRT-PCR was used to detect the expression levels of circ_0001273, circ_0018569, circ_0027152, and circ_0001273 which had the highest difference in expression in cancer and adjacent tissues was selected. siRNA was used for the knockdown of circ_0001273 in two types of OSCC cell lines, UM1 and CAL27, and the effects on the proliferation, migration, and invasion of UM1 and CAL27 cells were measured by MTS and Transwell experiments, respectively.
Results:The expression of circ_0001273 was abnormally increased in the 12 OSCC tissues (P < 0.05). After knocking down circ_0001273 in UM1 and CAL27 cells, the proliferation, migration and invasion abilities of UM1 and CAL27 cells were significantly reduced (P < 0.05).
Conclusion:The knockdown of circ_0001273 can inhibit the proliferation, migration and invasion of OSCC cells.
- Full text:敲低circ_0001273抑制口腔鳞癌细胞增殖、迁移及侵袭的研究.pdf
