Protective effects of hydrogen sulfide on diaphragmatic muscle of Type 1 diabetic rats and its anti-apoptotic mechanisms.
10.11817/j.issn.1672-7347.2015.11.002
- Author:
Rui YANG
1
;
Qiang JIA
1
;
Xiaolei GUO
1
;
Xiaofen LIU
1
;
Shanfeng MA
1
;
Qin GAO
1
;
Sudong GUAN
1
Author Information
1. Department of Physiology, Bengbu Medical College, Bengbu Anhui 233030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Diabetes Mellitus, Experimental;
Diaphragm;
drug effects;
Hydrogen Sulfide;
pharmacology;
Male;
Malondialdehyde;
metabolism;
Muscle Contraction;
drug effects;
Oxidative Stress;
Rats;
Rats, Sprague-Dawley;
Sulfides;
Superoxide Dismutase;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2015;40(11):1173-1178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the protective effects of hydrogen sulfide (H2S) on diaphragmatic muscle of Type 1 diabetic rats and its anti-apoptotic mechanism.
METHODS:Thirty male Sprague Dawley rats were randomly divided into a control group, a diabetes group and a treatment group (n=10 per group). Streptozotocin (i.p.) was utilized to establish a rat model of Type 1 diabetes mellitus (DM). The DM rats were treated with NaHS solution (i.p.). After 8 weeks, the diaphragmatic muscle contractility was assessed by isolated diaphragmatic strips experiments. The peak twitch tension (Pt), maximum tetanic tension (Po), time to peak contraction (CT), half relaxation time (1/2RT) and maximal rates of contraction/relaxation (±dT/dtmax) were measured. The alterations of diaphragmtic ultrastructure were observed by electron microscopy. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and caspase-3 were analyzed by spectrophotometric method. The expressions levels of Bcl-2 and Bax mRNA in diaphragmatic muscle were detected by RT-PCR.
RESULTS:Compared with the control group, in the diabetic group, the Pt, Po and ±dT/dtmax were significantly reduced (all P<0.01), while CT and 1/2RT were significantly increased (both P<0.01); ultrastructure in the diaphragmatic muscle were obviously changed; the content of MDA and the activity of caspase-3 were increased (both P<0.01), while the activity of SOD was decreased (P<0.01); the ratio of Bcl-2/Bax at mRNA level was decreased (P<0.01). Compared with the diabetes group, in the treatment group, the diaphragm contractility and ultrastructural damage were improved; the content of MDA and the activity of caspase-3 were decreased (P<0.05, P<0.01 respectively), while the activity of SOD was increased (P<0.01), the ratio of Bcl-2/Bax at mRNA level was also increased (P<0.01).
CONCLUSION:The exogenous H2S can protect diaphragmatic muscle of Type 1 diabetic rats, which is related to reducing oxidative damage and suppressing cell apoptosis.
