Injection of submandibular gland with recombinant Exendin-4 and adeno-associated virus for the treatment of diabetic rats.
10.11817/j.issn.1672-7347.2015.11.003
- Author:
Junhong WANG
1
;
Jiao WEN
2
;
Dan BAI
3
;
Yonghong GUO
4
Author Information
1. Department of Endocrinology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
2. Department of Oncology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
3. Department of Biochemistry and Molecular Biology, Medical College, Xi'an Jiaotong University, Xi'an 710004, China.
4. Department of Infecious Diseases, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Glucose;
analysis;
Dependovirus;
Diabetes Mellitus, Experimental;
therapy;
Diabetes Mellitus, Type 2;
therapy;
Exenatide;
Genetic Therapy;
Injections;
Insulin;
chemistry;
Peptides;
genetics;
therapeutic use;
Rats;
Rats, Sprague-Dawley;
Real-Time Polymerase Chain Reaction;
Recombinant Proteins;
genetics;
therapeutic use;
Submandibular Gland;
chemistry;
Venoms;
genetics;
therapeutic use;
alpha-Amylases;
chemistry
- From:
Journal of Central South University(Medical Sciences)
2015;40(11):1179-1185
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the therapeutic effects of recombinant Exendin-4 and double-stranded adeno-associated virus (Exendin-4/dsAAV) on SD rats with type 2 diabetes (T2DM) through injecting it into submandibular gland (SG).
METHODS:The Exendin-4/dsAAV was injected into submandibular gland of diabetic rat. The insulin and α-amylase were detected by real-time PCR at the 2nd, 4th and 8th weeks. The immunohistochemisty was used to detect the insulin contents in SG at the 8th week. The concentration of blood glucose and levels of insulin secretion were detected after pancreatectomy.
RESULTS:The SG gland was bigger in Exendin-4/dsAAV group than that in the control group, but the changes in α-amylase were not significant. The Exendin-4 and insulin gene expression was increased in the Exendin-4/dsAAV group (P<0.05). The Exendin-4 and insulin were positive in the SG. The blood glucose was lower and insulin concentration was higher in the Exendin-4/dsAAV group than those in the control group after pancreatectomy (P<0.05), and the insulin content was also increased in the dsAAV groups.
CONCLUSION:Continuous expression of Exendin-4 in SG may improve glucose control and insulin secretion in T2DM rats through inducing expression of insulin.
