Potentiation of cisplatin induced apoptosis by low molecular weight heparin in human hepatocellular carcinoma cell.
10.11817/j.issn.1672-7347.2015.12.003
- Author:
Wenjuan WU
1
;
Beikai YU
2
;
Yinghua GENG
3
Author Information
1. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu Anhui 233030, China wuwj_2012@126.com.
2. Department of Clinical Laboratory, Weifang Traditional Chinese Hospital, Weifang Shandong 261041, China.
3. Department of Hematology, First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Carcinoma, Hepatocellular;
pathology;
Cell Line, Tumor;
drug effects;
Cisplatin;
pharmacology;
Flow Cytometry;
Heparin, Low-Molecular-Weight;
pharmacology;
Humans;
Liver Neoplasms;
pathology;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Real-Time Polymerase Chain Reaction;
bcl-2-Associated X Protein;
metabolism;
fas Receptor;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2015;40(12):1298-1305
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of low molecular weight heparin (LMWH) on cisplatin (DDP)- induced apoptosis in human hepatocellular carcinoma cell and the underlying mechanisms.
METHODS:Hepatocellular carcinoma SMMC-7721 cells were divided into a control group, a LMWH group, a DDP group and a LMWH plus DDP group. The effect of the drugs on proliferation of hepatocellular carcinoma SMMC-7721 cells were evaluated by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay, and the apoptosis were detected by acridine orange/ethidium bromide (AO/EB) double fluorescence method and flow cytometry method. The expression levels of apoptosis-related protein Fas, Bcl-2 and Bax were detected by quantitative real-time PCR and Western blot.
RESULTS:Compared with the control group, the proliferation of SMMC-7721 cells was inhibited in the DDP group and the LMWH plus DDP group (both P<0.05). The mRNA and protein levels of Fas, Bcl-2, Bax in the SMMC-7721 cells were not obviously changed in the LMWH group (all P>0.05). The Fas level was increased obviously (P<0.05), while the Bcl-2 level moderately reduced (P<0.05), Bax were not obviously changed in the DDP group (P>0.05). Compared with the control group and the DDP group, the Bcl-2 level was reduced significantly in the LMWH plus DDP group (both P<0.05), while the level of Bax was increased obviously (both P<0.05). Compared with control group, the Fas level was increased significantly in the LMWH plus DDP group (P<0.05), but the increase was not significant compared with the DDP group (P>0.05).
CONCLUSION:LMWH can enhance the cisplatin-induced apoptosis in SMMC-7721 cells, which might be related to activation of mitochondrial pathway.
