Expression of interferon-induced protein with tetratricopetide repeats 1 and liver cell apoptosis in
mice with severe burns.
10.11817/j.issn.1672-7347.2015.06.005
- Author:
Xiaoshu GUO
1
,
2
,
3
;
Jiping GONG
4
;
Song WANG
3
,
5
;
Yuhui HAO
3
,
5
;
Yongli CHANG
6
;
Chen LI
6
Author Information
1. Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000
2. State Key Laboratory of Trauma, Burns and Combined Injury
3. Institute of Combined Injury, College of Military Preventive Medicine, Th ird Military Medical University, Chongqing 400038,China.
4. Department of Anesthesiology, Affi liated Central Hospital of Xinxiang Medical University, Xinxiang Henan 453000, China.
5. State Key Laboratory of Trauma, Burns and Combined Injury
6. Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Burns;
metabolism;
Carrier Proteins;
metabolism;
Caspase 3;
metabolism;
Caspase 8;
metabolism;
Hepatocytes;
cytology;
In Situ Nick-End Labeling;
Liver;
cytology;
Mice;
Mice, 129 Strain;
Mice, Inbred C57BL;
RNA-Binding Proteins
- From:
Journal of Central South University(Medical Sciences)
2015;40(6):598-604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between the expression of interferon-induced protein with tetratricopetide repeats 1 (IFIT1) and liver cell apoptosis in the acute stress period after severe burns.
METHODS:A total of 25 C57/129 adult mice were randomly divided into the normal control group (0 h) and the groups at 1, 6, 12 or 24 after severe burns (n=5 per group). A model with third degree (20% of the total body surface area) burn injury was established and then liver tissues were taken. IFIT1 expression was examined by Western blot. The expression of caspase-3 and -8 was measured by immunohistochemistry. Liver cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL).
RESULTS:After burns, IFIT1 expression was increased at 1 h, which reached the highest level at
6 h followed by a decrease at 12 h, which reached minimum level at 24 h. The differences between groups were significant (P<0.01). The caspase-3 and -8 levels significantly increased after burns in a time-dependent manner (P<0.01). Although at 0 h and 1 h there was no significant increase in liver cell apoptosis, the increase reached significance from 6 h to 24 h (P<0.01).
CONCLUSION:The increase in IFIT1 expression after severe burns promotes liver cell apoptosis.
