Ultrasound molecular detection of immediately blood-mediated inflammatory reaction induced by
islets transplantation in vitro.
10.11817/j.issn.1672-7347.2015.06.010
- Author:
Feng GAO
1
,
2
;
Qi LIANG
3
;
Junling LI
3
;
Qiong DONG
3
;
Xiaoqian MA
3
;
Wei WANG
3
Author Information
1. Power Metallurgy Research Institute, Central South University, Changsha 410083
2. Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
3. Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Contrast Media;
Humans;
Inflammation;
diagnostic imaging;
Islets of Langerhans Transplantation;
Microbubbles;
Thrombosis;
diagnostic imaging;
Ultrasonography
- From:
Journal of Central South University(Medical Sciences)
2015;40(6):632-638
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the feasibility of ultrasonic molecular imaging of immediately blood-mediated inflammatory reaction (IBMIR) in vitro.
METHODS:IBMIR models in vitro were divided into 3 groups: Group A, no microbubbles were added; Group B, non-targeted micro-bubbles were added; Group C, Lys-Gly-Asp-Ser (KGDS)-targeted microbubbles (MBK) were added. The ultrasonic enhancement of IBMIR in loops by ultrasonic contrast imaging was evaluated.
RESULTS:The contrast-enhanced US imaging did not show thrombus formation in the group A, whereas the thrombus was found in the Group B and C with a change in filling defects or ring enhancement, respectively. The time for detecting thrombosis was (7.3 ± 0.5) min and (13.2 ± 0.6) min in Group B and Group C, respectively (P<0.05). The average-gray scales of thrombus in Group B and Group C were 31.22 ± 3.56 and 75.85 ± 5.21, respectively (P<0.05). The fluorescence microscope also showed that MBK was attached to thrombus surrounding islets.
CONCLUSION:IBMIR model in vitro showed that KGDS-targeted ultrasound contrast agent could adhere to thrombus shell surrounding islets and molecular target ultrasonography could image these thrombi noninvasively and effectively.
