Fibroblast growth factor-1 inhibits Wnt/β-catenin pathway during adipogenesis.
10.11817/j.issn.1672-7347.2015.08.004
- Author:
Xiao LUO
1
;
Ru JIA
1
;
Ke LI
1
;
Xiaoying ZHU
1
;
Danwen ZHAO
1
;
Jonathan P WHITEHEAD
2
;
Jianqun YAN
1
Author Information
1. Department of Physiology and Pathophysiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
2. Metabolic Program, Mater Research Institute - University of Queensland, Translational Research Institute, Brisbane Queensland 4102, Australia.
- Publication Type:Journal Article
- MeSH:
Adipocytes;
drug effects;
metabolism;
Adipogenesis;
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors;
metabolism;
Cell Differentiation;
Cells, Cultured;
Fibroblast Growth Factor 1;
pharmacology;
Humans;
Recombinant Proteins;
pharmacology;
Transcription Factor 4;
Transcription Factors;
metabolism;
Wnt Signaling Pathway;
beta Catenin;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2015;40(8):843-850
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the time course and potential mechanism of fibroblast growth factor-1 (FGF-1) in the regulation of adipogenesis.
METHODS:We cultured human Simpson-Golabi-Behmel syndrome (SGBS) pre-adipocytes with recombinant FGF-1 and harvested cells at various stages prior to and during differentiation; at cell proliferation (D-3), confluence (D0), early (D3), middle (D7) and mature (D14) stages of differentiation. We determined lipid accumulation in mature adipocytes by morphological observation and quantitative measurement of oil red O staining. We also examined the expression of adipogenic genes and related markers involved in the Wnt/β-catenin pathway using quantitative Real-time PCR and Western blot.
RESULTS:Compared to control SGBS cells, treatment with FGF-1 increased lipid accumulation; induced a sustained increase in the mRNA for peroxisome proliferater-activated receptor γ (PPARγ), glyceraldehyde-3-phosphate dehydrogenase (G3PDH), adiponectin and glucose transporter type 4 (GLUT4); and promoted a sustained decrease in expression of markers of the Wnt/β-catenin pathway, β-catenin and transcription factor 4 (TCF4).
CONCLUSION:The adipogenic effects of FGF-1 are apparent throughout the whole priming and differentiation period in human SGBS pre-adipocytes. Furthermore, our results suggest that FGF-1
promotes adipogenesis, at least in part, via a sustained decrease in activity of the Wnt/β-catenin pathway.
