Angiopoietin-like 4 modulates aldolase A expression in human melanoma cell in a PKC dependent manner.
10.11817/j.issn.1672-7347.2015.08.005
- Author:
Yang SUN
1
;
Xiancheng WANG
1
;
Borong FANG
1
;
Xiang XIONG
1
;
Jianhong LONG
2
Author Information
1. Department of Burn and Plastic Surgery, Second Xiangya Hospital, Central South University, Changsha 410013, China.
2. Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Angiopoietin-like 4 Protein;
Angiopoietins;
genetics;
metabolism;
Blotting, Western;
Cell Line, Tumor;
Fructose-Bisphosphate Aldolase;
metabolism;
Gene Knockdown Techniques;
Humans;
Melanoma;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2015;40(8):851-857
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell.
METHODS:Overexpression or knockdown of ANGPTL4 was performed in WM-115 or WM-266-4 cells, respectively. The expression of ANGPTL4 and ALDOA was measured by RT-PCR and Western blot, respectively. The promoter activity of ALDOA gene was determined by luciferase assay.
RESULTS:The promoter activity of ALDOA gene and the expression of ALDOA (mRNA and protein) were increased or decreased in the melanoma cells with overexpression or knockdown of ANGPTL4, which was blocked by selective protein kinase C (PKC) inhibitor or restored by PKC agonist, respectively.
CONCLUSION:ANGPTL4 promotes ALDOA expression in human melanoma cell in a PKC dependent manner.
