Effect of dexmedetomidine on high-mobility group box 1 protein in rats with sepsis.
10.11817/j.issn.1672-7347.2015.09.007
- Author:
Xin LI
1
;
Jinghui LI
1
Author Information
1. Department of Intesive Care Unit, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Dexmedetomidine;
pharmacology;
HMGB1 Protein;
blood;
metabolism;
Lipopolysaccharides;
Male;
Rats;
Rats, Wistar;
Sepsis;
drug therapy;
metabolism;
Spleen;
drug effects;
metabolism;
Tumor Necrosis Factor-alpha;
blood
- From:
Journal of Central South University(Medical Sciences)
2015;40(9):987-992
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of dexmedetomidine (DEX) on high-mobility group box 1 protein (HMGB1) in the serum and spleen in rats with sepsis and the underlying mechanisms.
METHODS:A total of 100 male Wistar rats were randomly divided into five groups: a control group, a sepsis group, a DEX group, an α-bungarotoxin- tetramethylrhodamine (α-BGT) plus DEX group and an α-BGT group (n=20 in each group). In the control group, same volume of normal saline (NS) was administrated, while lipopolysaccharide (LPS) was injected to the vein to develop a classic sepsis model. At the 48th h after LPS injection, the rats were sacrificed, and the blood and spleen were collected. The content of tumor necrosis factor-α (TNF-α) and HMGB1 in blood serum were detected by enzyme-linked immuno sorbent assay (ELISA), and the HMGB1 in the spleen was examined by Western blot.
RESULTS:Among the 5 groups, there were statistical significance in the mortality, the serum level of TNF-α and HMGB1, and the content of secreting type of HMGB1 in spleen (all P<0.05), and the serum level of HMGB1 was positively correlated with the content of secreting type of HMGB1 (r=0.863, P<0.05).
CONCLUSION:DEX exert late anti-inflammatory effects in the serum and spleen in rats with sepsis, which is related to the activation of the cholinergic anti-inflammatory pathway.
