Identification of miR-126 knockdown mouse and the change of blood glucose.
10.11817/j.issn.1672-7347.2015.01.003
- Author:
Yan HU
1
;
Yongju LI
;
Chao CHEN
;
Shunfei ZHU
;
Mengmeng GUO
;
Sijing LIU
;
Jing ZHENG
;
Nalin QIN
;
Lin XU
Author Information
1. Department of Immunology and Immunology Innovation Base of Postgraduate Education, Zunyi Medical College, Zunyi Guizhou 563000, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Glucose;
analysis;
Body Weight;
Diabetes Mellitus, Type 2;
Gene Knockdown Techniques;
Liver;
pathology;
Mice;
MicroRNAs;
genetics;
Pancreas;
pathology;
Real-Time Polymerase Chain Reaction
- From:
Journal of Central South University(Medical Sciences)
2015;40(1):12-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expression of miR-126 in different tissues and organs and the change of peripheral blood glucose in microRNA-126 knock down (miR-126 KD) mouse, and to explore the pathological significance.
METHODS:Total RNAs were isolated from twelve kinds of tissues and organs in wild-type mouse (WT) and miR-126 KD mouse respectively. Th en, the expression level of miR-126 was detected by real-time PCR assay. Th e levels of peripheral blood glucose and body weight of miR-126 KD mice were measured. Th e pathologic changes of pancreas and lung tissue were observed by HE staining.
RESULTS:Compared with the WT mice, the relative expression of miR-126 in spleen, liver, muscle and lung from the miR-126 KD mice were dramatically decreased respectively (P<0.05). The level of peripheral blood glucose in the miR-126 KD mouse increased significantly at seven week and sixteen week after the birth (P<0.05). HE staining showed that the pathological structure of pancreas and liver were abnormal. The body weight of miR-126 KD mice was increased obviously from thirteen week after birth (P<0.05).
CONCLUSION:Peripheral blood glucose levels in the miR-126 KD mouse were dramatically elevated, which might be related to the pathological changes in the structure of pancreas and liver. These results suggest that miR-126 may play an important role in the metabolism of blood glucose and the development of type 2 diabetes mellitus.
