Grape seed proanthocyanidins extract inhibits pancreatic cancer cell growth through down-regulation of miR-27a expression.
10.11817/j.issn.1672-7347.2015.01.008
- Author:
Jia MA
1
;
Binbin FANG
;
Fanpeng ZENG
;
Haijie PANG
;
Cong MA
;
Jun XIA
Author Information
1. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu Anhui 233030, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
drug effects;
Cell Movement;
Cell Proliferation;
Down-Regulation;
Grape Seed Extract;
pharmacology;
Humans;
MicroRNAs;
genetics;
metabolism;
Pancreatic Neoplasms;
pathology;
Proanthocyanidins;
pharmacology;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2015;40(1):46-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the eff ect of grape seed proanthocyanidins extract (GSPE) on the growth of pancreatic cancer cells and the underlying mechanisms.
METHODS:The pancreatic cancer AsPC-1 cells were cultured in vitro. The effects of GSPE on cell proliferation, apoptosis and migration were analyzed by MTT, Annexin V-FITC/PI and Transwell migration assay, respectively. The expression of miR-27a and FOXO1 in AsPC-1 cells was determined by real-time RT-PCR and Western blot, respectively. The miR-27a inhibitors were applied to verify the role of miR-27a in mediation of GSPE effects.
RESULTS:GSPE inhibited cell growth in a dose-dependent manner. This inhibitory effect was significant when the dosage of GSPE was more than 50 μg/mL (P<0.05 vs control). GSPE also could induce apoptosis and inhibit cell migration. MiR-27a expression was notably down-regulated when the dosage of GSPE was 75 μg/mL (P<0.01 vs control). Compared with the control group, cell proliferation inhibition was significantly increased in the miR-27a inhibitor group, the GSPE group and the miR-27a inhibitor plus GSPE group (P<0.01), while cell migration was significantly decreased (P<0.01). Compared with the GSPE or the miR-27a inhibitor group, the growth and migration inhibitory effects in the miR-27a inhibitor plus GSPE group were more obviously (P<0.01). Both GSPE and miR-27a inhibitor alone could up-regulate FOXO1 expression. But these effects were more apparent when they are applied in combination.
CONCLUSION:GSPE inhibites AsPC-1 cells' growth and migration partly through down-regulation of miR-27a expression.
