Biological characteristics of human olfactory mucosa mesenchymal stem cells.
10.11817/j.issn.1672-7347.2015.01.009
- Author:
Lite GE
1
,
2
;
Yi ZHUO
;
Da DUAN
;
Zhenyu ZHAO
;
Xiaohua TENG
;
Lei WANG
;
Ming LU
Author Information
1. Department of Neurosurgery, 163 Hospital of PLA
2. Second Affi liated Hospital, Hunan Normal University, Changsha 410003, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cells, Cultured;
Humans;
Mesenchymal Stem Cells;
cytology;
ultrastructure;
Microscopy, Electron, Scanning;
Microscopy, Electron, Transmission;
Olfactory Mucosa;
cytology
- From:
Journal of Central South University(Medical Sciences)
2015;40(1):53-58
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the biological characteristics of the human olfactory mucosa mesenchymal stem cells (hOM-MSCs).
METHODS:The hOM-MSCs were isolated, cultured and identified in vitro. Scanning electron microscope and transmission electron microscope were used to observe the ultrastructure of hOMMSCs. Th e cells were induced towards adipocyte, osteocyte, neural stem cells, neural-like-cells in vitro.
RESULTS:The hOM-MSCs were mainly in spindle shape, arranged with radial colony. The hOMMSCs expressed CD73 and CD90 but no CD34 and CD45. Th e short and thick microvilli processes were seen at the surface of hOM-MSCs by scanning electron microscope, and 2 different cellular morphology of hOM-MSCs were seen under transmission electron microscope. Moreover, the hOMMSCs could be differentiated into adipocyte, osteocyte, neural stem cells and neural cells.
CONCLUSION:The hOM-MSCs possess general biological characteristics of MSCs and display multiple differentiation functions. They can be served as ideal seed cells in tissue-engineering for injury repair.
