Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats.
10.11817/j.issn.1672-7347.2015.02.005
- Author:
Chenggui MIAO
1
,
2
,
3
;
Guoliang ZHOU
;
Meisong QIN
;
Jianzhong CHEN
;
Chengfeng LI
;
Huaqi HE
Author Information
1. Department of Pharmaceutics, Food and Drug College, Anhui Science and Technology University , Fengyang Anhui 233100
2. Department of Basic and Clinical Pharmacology, College of Pharmacy, Anhui Medical University, Heifei 230032
3. Poultry Disease Monitoring Anhui Key Laboratory, Anhui Science and Technology University, Fengyang Anhui 233100, China fensheng1981@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Arthritis, Experimental;
Caspase 3;
metabolism;
Fibroblasts;
cytology;
drug effects;
Interleukin-6;
metabolism;
Interleukin-8;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Pulsatilla;
chemistry;
Rats;
Rats, Sprague-Dawley;
Synovial Membrane;
cytology;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2015;40(2):144-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.
METHODS:A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
RESULTS:Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).
CONCLUSION:PULC may inhibit the FLS proliferation in AA rats by increase in FLS apoptosis.
