Effects of apigenin on self-renewal and
uPAR expression in NCI-H446 cell line.
- Author:
Ling SHU
1
;
Qing YUAN
1
;
Yinghong CUI
1
;
Shuwen SUN
1
;
A CHEN
1
;
Dan CHEN
1
;
Jianguo CAO
1
;
Jiansong ZHANG
1
Author Information
1. Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Apigenin;
pharmacology;
Cell Line, Tumor;
Down-Regulation;
drug effects;
genetics;
Humans;
Lung Neoplasms;
Neoplastic Stem Cells;
drug effects;
pathology;
physiology;
Receptors, Cell Surface;
Receptors, Urokinase Plasminogen Activator;
drug effects;
genetics;
metabolism;
Signal Transduction;
Small Cell Lung Carcinoma;
drug therapy;
pathology;
Spheroids, Cellular;
drug effects;
physiology;
Stem Cells
- From:
Journal of Central South University(Medical Sciences)
2016;41(11):1124-1127
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of apigenin on self-renewal for sphere-forming cells in human small cell lung cancer cell line NCI-H446 and the underlying mechanisms.
Methods: Sphere-forming cells from NCI-H446 cell line were cultured in stem cell-conditioned culture medium with ultra-low attachment surface plates. The rate of sphere-forming cells in the second passage sphere-forming cells was used to evaluate the inhibitory effects of apigenin on the self-renewal for sphere-forming cells. The protein level of urokinase-type plasminogen activator receptor (uPAR) in spheroids was analyzed by Western blot.
Results: Apigenin signifcantly inhibited the self-renewal of the second passage sphere-forming cells [0, 5.0, 10.0, 20.0 μmol/L apigenin: (18.2±1.9)%, (13.6±1.7)%, (10.6±1.6)%, (6.9±1.3)%, respectively] and down-regulated uPAR expression in a concentration-dependent manner (P<0.05).
Conclusion: Apigenin inhibits the self-renewal capacity of sphere-forming cells in NCI-H446 cells, which may be associated with down-regulation of uPAR expression.
