Effect of prolonged propofol infusion on myocardial enzyme, mitochondrial cytochrome C and adenosine
triphosphate in rabbits.
- Author:
Guangmin XU
1
;
Zhixun LAN
1
;
Xianxiang TONG
1
Author Information
1. Department of Anesthesia, Sichuan Academy of Medical Sciences/Sichuan Provincial People's Hospital, Chengdu 610072, China.
- Publication Type:Journal Article
- MeSH:
Adenosine Triphosphate;
metabolism;
Animals;
Creatine Kinase;
blood;
metabolism;
Creatine Kinase, MB Form;
blood;
metabolism;
Cytochromes c;
metabolism;
Emulsions;
administration & dosage;
pharmacology;
Infusions, Intravenous;
Mitochondria;
drug effects;
Myocardium;
chemistry;
enzymology;
Phospholipids;
administration & dosage;
pharmacology;
Polyphosphates;
Propofol;
administration & dosage;
pharmacology;
Rabbits;
Soybean Oil;
administration & dosage;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2016;41(11):1181-1185
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of long-time propofol infusion on myocardial enzymes, mitochondrial cytochrome C and ATP in rabbits.
Methods: A total of 18 New Zealand rabbits were randomly divided into 3 groups: a control group, a propofol group and an intralipid group. The rabbits were continuously infused with 0.9% normal saline in the control group, 1% propofol in the propofol group, and 10% intralipid in the intralipid group, respectivey. The arterial blood was collected at 0, 8, 16 h and the end of experiment to examine creatine kinase (CK) and creatine kinase isoenzyme (CK-MB). In the end, the myocardial mitochondria from myocardial tissues was separated by differential centrifugation, and mitochondrial cytochrome C content and adenosine triphosphate (ATP) levels were examined by high performance liquid chromatography.
Results: Compared with the control group, the release of cytochrome C from mitochondria were increased in the propofol group and the intralipid group (both P<0.05), but there was no significant difference between them (P>0.05). There was also no significant difference in the ATP content of the mitochondria among the 3 groups (P>0.05). The levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group and the intralipid group compared with that before the infusion (all P<0.05); compared with the control group, the levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group and the intralipid group (all P<0.05); compared with the intralipid group, the levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group (all P>0.05); compared with the control group, the levels of CK-MB were obviously increased in the infusion of propofol for 24 h in the propofol group (P<0.05).
Conclusion: The levels of serum CK increase after the infusion of propofol and intralipid for a long time, and the levels of CK-MB also elevate in the infusion of propofol. Propofol and intralipid can increase the release of myocardial mitochondrial cytochrome C, but they don't affect the ATP production in myocardial mitochondrial.
