Hypoxic condition promotes olfactory mucosa mesenchymal stem cells to differentiate into neurons and
underlying mechanisms.
10.11817/j.issn.1672-7347.2016.12.002
- Author:
Yi ZHUO
1
;
Ting YUAN
1
;
Da DUAN
1
;
Lei WANG
1
;
Lite GE
1
;
Pei WU
1
;
Hao WANG
1
;
Ming LU
1
Author Information
1. Department of Neurosurgery, 163 Hospital of PLA/ Second Affiliated Hospital, Hunan Normal University, Changsha 410003, China.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Cell Differentiation;
physiology;
Cells, Cultured;
Culture Media, Conditioned;
chemistry;
pharmacology;
Flow Cytometry;
Glial Fibrillary Acidic Protein;
metabolism;
Hypoxia;
physiopathology;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Indazoles;
pharmacology;
Mesenchymal Stem Cells;
physiology;
Neurogenesis;
physiology;
Neuroglia;
metabolism;
physiology;
Neurons;
physiology;
Olfactory Mucosa;
Potassium Channels;
Signal Transduction;
Tubulin;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2016;41(12):1252-1259
- CountryChina
- Language:Chinese
-
Abstract:
To explore whether hypoxic condition could promote the olfactory mucosa mesenchymal stem cells (OM-MSCs) to differentiate into neurons with the olfactory ensheathing cells (OECs) supernatant and the potential mechanisms.
Methods: The OM-MSCs and OECs were isolated and cultured, and they were identified by flow cytometry and immunofluorescence. The OM-MSCs were divided into three groups: a 3%O2+ HIF-1α inhibitors (lificiguat: YC-1) + OECs supernatant group (Group A) , a 3%O2 + OECs supernatant group (Group B) and a 21%O2 + OECs supernatant group (Control group). The neurons, which were differentiated from OM-MSCs, were assessed by immunofluorescence test. The mRNA and protein expression of hypoxia-inducible factor-1α (HIF-1α), βIII-tubulin and glial fibrillary acidic portein (GFAP) were detected by quantitative polymerase chain reaction (Q-PCR) and Western blot. The potassium channels were analyzed by patch clamp.
Results: The neurons differentiated from OM-MSCs expressed the most amount of βIII-tubulin, and the result of Q-PCR showed that HIF-1α expression in the Group B was significantly higher than that in the other groups (all P<0.05). Western blot result showed that the βIII-tubulin protein expression was significantly higher and GFAP protein expression was obviously decreased in the Group B (both P<0.05). The patch clamp test confirmed that the potassium channels in the neurons were activated.
Conclusion: Hypoxic condition can significantly increase the neuronal differentiation of OM-MSCs by the OECs supernatant and decrease the production of neuroglia cells, which is associated with the activation of HIF-1 signal pathway.
