BM-MSCs from Wuzhishan mini-pigs delay the progress of renal fibrosis induced by chronic kidney disease through
autocrine hepatocyte growth factor in vitro.
10.11817/j.issn.1672-7347.2016.12.003
- Author:
Yang XIANG
1
;
Jiale LONG
1
;
Jiansheng XING
1
;
Yuanhui GAO
2
;
Qing CHENG
1
;
Yong CAI
3
;
Zhenxiang LIU
1
;
Shufang ZHANG
2
;
Lie CHEN
4
;
Chao YANG
5
;
Zhiming BAI
1
Author Information
1. Department of Urology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
2. Department of Central Laboratory, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
3. Department of Urology, Second People's Hospital of Chengdu, Chengdu 610000, China.
4. Department of Ultrasound, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
5. Laparoscopic Center, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Autocrine Communication;
physiology;
Bone Marrow Cells;
Cells, Cultured;
Fibrosis;
physiopathology;
prevention & control;
Hepatocyte Growth Factor;
metabolism;
Kidney;
drug effects;
pathology;
physiopathology;
Mesenchymal Stem Cells;
drug effects;
Renal Insufficiency, Chronic;
complications;
physiopathology;
Swine;
Swine, Miniature;
Ureteral Obstruction;
complications
- From:
Journal of Central South University(Medical Sciences)
2016;41(12):1260-1269
- CountryChina
- Language:Chinese
-
Abstract:
To isolate bone marrow mesenchymal stem cells (BM-MSCs) and establish the model of chronic kidney disease (CKD) of Wuzhishan (WZS) mini-pig, and to study the repairment effect of BM-MSCs on CKD-induced renal fibrosis in vitro.
Methods: Density gradient method was used to isolate and culture BM-MSCs. The cells were verified by morphology, phenotype, differentiation and so on. The left partial ureteral obstruction (LPUUO) was used to establish the CKD model, which was evaluated by B-ultrasound, single-photon emission computed tomography (SPECT), HE and Masson staining. The cells were divided into 3 groups, the tissue plus BM-MSCs group, the tissue group, and the BM-MSCs group, respectively. Seven days later, the supernatants were collected to observe the changes of hepatocyte growth factor (HGF) cumulative release. HE and Masson staining was used to observe the changes of renal tissue.
Results: The isolated BM-MSCs possessed the features as follow: fibroblast-like adherent growth; positive in CD29 and CD90 expression while negative in CD45 expression; osteogenic induction and alizarin red staining were positive; alcian blue staining were positive after chondrogenic induction. Twelve weeks after the operation of LPUUO, B-ultrasound showed the thin renal cortical with pelvis effusion; SPETCT showed the left kidney delayed filling and renal impairment. The accumulation of HGF in the tissue plus BM-MSCs group was significantly higher than that in the tissue alone group at the 1st, 5th, 6th, 7th day, respectively (P<0.05). HE staining showed the different degree of renal lesions between the tissue plus BM-MSCs+CKD group and the tissue alone group, which was aggravated with the time going. Masson staining showed that the cumulative optical density of blue-stained collagen fibers in tissue plus BM-MSCs group was significantly lower than that in the tissue group at the 5th to 7th day (P<0.05).
Conclusion: BM-MSCs from WZS mini-pig can inhibit or delay the progress of CKD-induced renal fibrosis through autocrine HGF in vitro.
