Effects of laser solid forming of porous titanium on proliferation of osteoblast and RANKL/OPG expression.
10.11817/j.issn.1672-7347.2016.12.005
- Author:
Hui CHEN
1
;
Shanshan DU
2
;
Ping ZHEN
1
;
Xusheng LI
1
;
Xiaoyan LIANG
1
;
Lijuan FAN
1
;
Jie JIANG
1
;
Haiou YANG
3
;
Jun LIU
1
Author Information
1. Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
2. Physical Examination Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
3. State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi'an 710072, China.
- Publication Type:Journal Article
- MeSH:
Biocompatible Materials;
chemistry;
Cell Adhesion;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Culture Media;
chemistry;
Ligands;
Osteoblasts;
drug effects;
Osteogenesis;
drug effects;
Osteoprotegerin;
metabolism;
Porosity;
Receptor Activator of Nuclear Factor-kappa B;
metabolism;
Tissue Engineering;
instrumentation;
Tissue Scaffolds;
chemistry;
Titanium;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2016;41(12):1278-1284
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the effect of laser solid forming (LSF) of porous titanium on receptor activator of NF-κB ligand (RANKL)/osteoprorotegerin (OPG) expression and osteoblast cells growth.
Methods: The DMEM and sterile saline were used for porous titanium extract. The osteoblast cells were cultured in the extract while equal amount of DMEM and sterile saline were added to the control group. The growth of the cells were observed under an inverted phase contrast microscope. MTT was used to detect the growth inhibitory rates. The adhesion capacity of osteoblasts were measured. The growth in the material surface was examined by the electron microscope, and the expressions of RANKL and OPG were determined by Westen blot.
Results: At the first day, the osteoblast proliferation rate was significantly different (P<0.05), at the fourth and seventh day, the osteoblast proliferation rate was not significantly affected in the LSF group (P>0.05); at each time point, the osteoblast proliferation rate were significantly different between the two groups (P<0.05). Compared with the control group, RANKL and OPG protein expression were not significantly different (P>0.05). The laser solid forming of porous titanium showed well bone compatibility.
Conclusion: The porous titanium did not affect osteoblast proliferation due to its well bone compatibility. It did not affect the OPG/RANKL/RANK-axis system of bone metabolism, exibiting a wide applicable prospect for tissue engineering.
