MicroRNA-150 inhibits osteosarcoma cell proliferation by targeting RUNX2 gene.
10.11817/j.issn.1672-7347.2016.12.006
- Author:
Longfei WANG
1
;
Weiguo WANG
2
;
Jinsong LI
2
;
Shijie CHEN
2
;
Ruisen ZHAN
2
Author Information
1. Department of Orthopaedic Trauma, Tianjin Hospital, Tianjin 300211, China.
2. Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
3' Untranslated Regions;
Actins;
metabolism;
Bone Neoplasms;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
genetics;
Core Binding Factor Alpha 1 Subunit;
drug effects;
genetics;
pharmacology;
physiology;
Gene Expression Regulation, Neoplastic;
drug effects;
genetics;
Humans;
MicroRNAs;
pharmacology;
Osteoblasts;
physiology;
Osteosarcoma;
genetics;
physiopathology
- From:
Journal of Central South University(Medical Sciences)
2016;41(12):1285-1290
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the microRNA (miR)-150 expression level in human osteosarcoma cell lines (Saos-2, MG-63) and its function in cell proliferation, and to explore the potential molecular mechanisms.
Methods: MiR-150 expression levels in human osteosarcoma cell lines (Saos-2, MG-63) and normal osteoblast cell line (NHOst) were detected by relative quantitative real-time PCR (qRT-PCR). MiR-150 was overexpressed in Saos-2 and MG-63 cells by lentivirus infection. Cell proliferation rates were monitored by MTS assay. RUNX2 and β-actin protein levels were examined by Western blot. Inhibitory effect of miR-150 on binding RUNX2 3'-UTR was detected by Dual-Luciferase assay.
Results: MiR-150 expression level is lower in human osteosarcoma cell lines (Saos-2, MG-63) compared to the normal osteoblast cell line (NHOst) (0.23±0.02 and 0.32±0.03 vs 1.00±0.02), which showed statistical significance (P<0.01). After lentivirus infection, miR-150 level increased in Saos-2 (P<0.01) and MG-63 cells (P<0.01). Overexpression of miR-150 decreased cell proliferation and RUNX2 protein level in Saos-2 and MG-63 cells. The binding of miR-150 to RUNX2 3'-UTR decreased luciferase activity by 69% in Saos-2 cells (P<0.05) and 59% in MG-63 cells (P<0.05). Administration of exogenous RUNX2 recovered the cell proliferation in miR-150 overexpressed Saos-2 and MG-63 cell lines (P<0.01).
Conclusion: MiR-150 inhibites proliferation in human osteosarcoma cell lines through binding to RUNX2 3'-UTR, resulting in the reducion of RUNX2 protein level.
