Effect of calcium channel blockers on primary cultured human urethra scar fibroblasts.
10.11817/j.issn.1672-7347.2016.12.011
- Author:
Mingqiang ZENG
1
;
Junjie CHEN
1
;
Liang HUANG
1
;
Ruizhi XUE
1
;
Xuyu XIANG
1
;
Fanchang ZENG
1
;
Guilin WANG
1
;
Zhengyan TANG
1
Author Information
1. Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Calcium Channel Blockers;
pharmacology;
Cell Proliferation;
drug effects;
Cells, Cultured;
Cicatrix;
prevention & control;
Fibroblasts;
drug effects;
Humans;
Matrix Metalloproteinase 2;
drug effects;
Matrix Metalloproteinase 9;
drug effects;
Matrix Metalloproteinase Inhibitors;
pharmacology;
Up-Regulation;
drug effects;
Urethra;
cytology;
pathology;
Verapamil;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2016;41(12):1317-1322
- CountryChina
- Language:Chinese
-
Abstract:
To investigate effects of verapamil on primary cultured human urethral scar fibroblasts (USFs) and to provide basis for protecting the formation of urethra scar.
Methods: The cell proliferation was evaluated with the cell counting kit (CCK)-8 method after USFs were incubated various verapamil concentrations (50, 100, 150, 200, or 250 μmol/L) or solvent for 12, 24, or 48 h. The protein level of matrix metalloproteinase (MMP) was evaluated with ELISA after cells were incubated with verapamil (100 μmol/L) or solvent (control cells) for 24 h.
Results: The proliferation of USFs was obviously suppressed after verapamil treatment, which was in a dose-dependent and time-dependent manner. Meanwhile, the protein levels of MMP-2 and MMP-9 in the verapamil treatment group increased obviously compared with those of the control groups (P<0.05).
Conclusion: Calcium channel blockers may prevent the excessive formation of urethra scar by inhibiting the proliferation of urethral scar fibroblasts and enhancing the activity of MMP.
