MiR-519 inhibits gastric cancer cell activity through regulation of HuR expression.
10.11817/j.issn.1672-7347.2016.01.003
- Author:
Ning ZHOU
1
;
Yang ZHOU
2
;
Yong TANG
1
;
Wenyan YU
3
Author Information
1. Department of Gastroenterology, Xinjiang Medical University Affiliated Tumor Hospital, Xinjiang Medical University, Urumuqi 830000, China.
2. Department of Orthopaedics, Xinjiang Medical University Affiliated Tumor Hospital, Xinjiang Medical University, Urumuqi 830000, China.
3. Department Pathophysiology, College of Preclinical Medicine, Xinjiang Medical University, Urumuqi 830000, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
ELAV-Like Protein 1;
genetics;
metabolism;
Gene Expression Regulation, Neoplastic;
Humans;
MicroRNAs;
genetics;
metabolism;
Stomach Neoplasms;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2016;41(1):19-23
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of miR-519 in regulating the cell activity of gastric cancer.
METHODS:The expression of miR-519 was measured by qPCR. The protein expression of HuR was detected by Western blot. MTT assay was used to detect the cell activity of gastric cancer cells.
RESULTS:The protein level of HuR in the gastric cancer cells was higher than that in the control cells. Over-expression of HuR resulted in the increase in the cell activity of gastric cancer (P<0.001). Suppression of HuR decreased the cell activity of gastric cancer (P=0.001). The miR-519 level in the gastric cancer cells was lower than that in the control cells (P=0.001). Over-expression of miR-519 decreased the protein level of HuR and the activity of gastric cancer cells.
CONCLUSION:MiR-may inhibit the gastric cancer cell activity through suppression of HuR expression.
