Expression of LXR-β in human gastric cancer tissue and the effect of GW3965 on the proliferation of gastric cancer cell line SGC-7901.
10.11817/j.issn.1672-7347.2016.02.003
- Author:
Ran WANG
1
;
Ruixin LI
1
;
Qiaocheng WEN
1
;
Kun PENG
1
;
Xiangzhou TAN
1
;
Zhikang CHEN
1
Author Information
1. Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Activating Transcription Factor 4;
genetics;
metabolism;
Benzoates;
pharmacology;
Benzylamines;
pharmacology;
Cell Line, Tumor;
drug effects;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Gene Silencing;
Humans;
Liver X Receptors;
Orphan Nuclear Receptors;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
RNA, Small Interfering;
genetics;
Stomach Neoplasms;
pathology;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2016;41(2):127-133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the expression of liver X receptor-β (LXR-β) in human gastric cancer tissue, and to explore the effect of GW3965, an agonist of LXRs, on proliferation of gastric cancer cell line SGC-7901.
METHODS:The immunohistochemical assay was used to detect the expression of LXR-β, activating transcription factor 4 (ATF4) in gastric cancer tissues and the corresponding pericarcinoma tissues in 114 patients. Real-time quantitative PCR and Western blot were used to determine mRNA and protein levels of ATF4 and ATP-binding cassette 1 (ABCA1), one of the downstream target genes of LXRs, in SGC-7901 cells with or without GW3965 treatment. Cell counting kit-8 (CCK-8) assay was performed to detect cell proliferation. The expression of ATF4 was silenced by short hairpin RNA (shRNA).
RESULTS:The expressions of LXR-β and ATF-4 were obviously down-regulated in the gastric cancer tissues than that in the corresponding pericarcinoma tissues (both P<0.05). Compared with the control cells, GW3965 treatment inhibited proliferation of SGC-7901 cells and up-regulated ATF4 and ABCA1 expressions (both P<0.05). Knockdown of ATF4 can reverse the antiproliferative effect of GW3965 on SGC-7901 cells.
CONCLUSION:The expression of LXR-β is decreased in human gastric cancer tissues, and activation of LXRs by GW3965 could inhibit the proliferation of SGC-7901 cells via ATF4.
