Mechanisms for effect of osthole on inhibiting the growth and invasion of bladder cancer cells.
10.11817/j.issn.1672-7347.2016.04.002
- Author:
Jun LIU
1
;
Ran XU
1
;
Xiaokun ZHAO
1
Author Information
1. Department of Urology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
CD13 Antigens;
metabolism;
Caspase 3;
metabolism;
Cell Line, Tumor;
drug effects;
Coumarins;
pharmacology;
Cyclooxygenase 2;
metabolism;
ErbB Receptors;
metabolism;
Humans;
Matrix Metalloproteinase 2;
metabolism;
NF-kappa B;
metabolism;
Urinary Bladder Neoplasms;
metabolism;
pathology;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2016;41(4):345-352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of osthole on epidermal growth factor receptor tyrosine kinase (EGFR-TPK), matrix-metalloproteinase-2 (MMP-2), aminopeptidase N (APN) in bladder cancer cell and the underlying mechanism.
METHODS:The T24 cell lines were cultured. The inhibitory effects of osthole on EGFR-TPK, APN and MMP-2 were evaluated by spectrophotometric and MTT assay. The caspase-3 activity and the expression COX-2 and VEGF in T24 were examined. The activity of NF-κB was determined by electrophoretic mobility shift assay.
RESULTS:The half inhibition concentrations (IC50) of osthole on EGFR-TPK, APN and MMP-2 were (45.33±3.98), (28.21±3.23) and (8.11±0.54) µmol/L, respectively. The growth inhibitory rates for T24 cells were increased in a dose-dependent manner (P<0.05). The caspase-3 activities were significantly increased in T24 cells in the osthole group compared with control group, while the expression of angiogenesis related-protein COX-2, VEGF, and NF-κB in T24 cells were decreased.
CONCLUSION:Through the inhibitory effect on EGFR-TPK, APN and MMP-2, osthole can decrease COX-2, VEGF and NF-κB expression while increase the activity of caspase-3, eventually blocking the growth and invasion of bladder cancer cell.
