Effect of hydrogen sulfide on cardiac myosin light chain kinase expression in diabetic rats.
10.11817/j.issn.1672-7347.2016.04.003
- Author:
Rui YANG
1
;
Qiang JIA
2
;
Xiaofen LIU
2
;
Yuanyuan WANG
3
;
Qin GAO
2
;
Shanfeng MA
2
Author Information
1. Department of Physiology, Bengbu Medical College, Bengbu Anhui 233030, China.
2. Department of Physiology.
3. Center of Functional Experiment, Bengbu Medical College, Bengbu Anhui 233030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cardiotonic Agents;
pharmacology;
Creatine Kinase, MB Form;
blood;
Diabetes Mellitus, Experimental;
drug therapy;
Heart;
drug effects;
Hemodynamics;
Hydrogen Sulfide;
pharmacology;
L-Lactate Dehydrogenase;
blood;
Male;
Myocardium;
ultrastructure;
Myosin-Light-Chain Kinase;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Sulfides;
pharmacology;
Ventricular Function, Left;
drug effects
- From:
Journal of Central South University(Medical Sciences)
2016;41(4):353-358
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of hydrogen sulfide (H2S) on cardiac myosin light chain kinase (MLCK) expression in diabetic rats.
METHODS:A total of 32 male SD rats were randomly divided into a normal control group (NC group), a diabetic control group (DM), a NaHS treatment group (DM+NaHS) and a NaHS group (NaHS) (n=8 in each group). Intraperitoneal injection of streptozotocin was utilized to establish Type 1 diabetic rat model. The diabetic rats in the DM+NaHS and NaHS groups were intraperitoneally injected with 28 μmol/kg NaHS solution. Eight weeks later, the ventricular hemodynamic parameters, the ratio of heart weight/body weight (HW/BW ratio), the levels of lactate dehydrogenase (LDH) and creatine kinase MB isozyme (CK-MB) in serum were determined. The ultrastructures of myocardium were observed under electron microscopy. The expressions of MLCK mRNA and protein level in myocardium were detected by RT-PCR and Western blot, respectively.
RESULTS:Compared with the NC group, there was no significant difference in the various indexes in the NaHS group (all P>0.05). The function of left ventricular contract and relaxation were decreased obviously in diabetic rats, while the HW/BW ratio was increased (all P<0.01). The levels of LDH and CK-MB were increased (both P<0.01) in serum, while the levels of MLCK mRNA and protein were decreased significantly (both P<0.01) in myocardial tissues. Compared with the DM group, the left ventricular hemodynamic parameters and myocardial ultrastructure damage were improved in the DM+NaHS group, while the HW/BW ratio was decreased (all P<0.05). The levels of LDH and CK-MB were decreased (both P<0.01), while the levels of MLCK mRNA and protein were increased significantly (both P<0.01).
CONCLUSION:H2S can protect myocardium in diabetic rats, which may be associated with upregulation of cardiac MLCK.
