Expression of two transcript variants of long noncoding RNA C6orf176 in non-small cell lung cancer and its clinical significance.
10.11817/j.issn.1672-7347.2016.06.002
- Author:
Jun WANG
1
;
Zhenzi PENG
1
;
Yeping DONG
1
;
Dan HE
1
;
Chaojun DUAN
2
,
3
;
Xinying LI
4
Author Information
1. Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
2. Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008
3. Key Laboratory of Cancer Proteomics, National Health and Family Planning Commission, Changsha 410008, China.
4. Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Actins;
Area Under Curve;
Carcinoma, Non-Small-Cell Lung;
Cell Differentiation;
Down-Regulation;
Gene Expression Regulation, Neoplastic;
Humans;
Lung Neoplasms;
RNA, Long Noncoding;
ROC Curve;
Real-Time Polymerase Chain Reaction;
Smoking;
Transcription, Genetic;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2016;41(6):560-565
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore two transcript variants expression of long noncoding RNA C6orf176 in non-small cell lung cancer (NSCLC) and the clinical pathological significance.
METHODS:The expressions of transcript variant 1 (TV1) and transcript variant 2 (TV2) of long noncoding RNA C6orf176 in 57 NSCLC and adjacent cancerous tissues were examined by qPCR with β-actin as internal control.
RESULTS:Based on the results of qPCR, for C6orf176-TV1, 42 cases were down-regulated and 15 cases were up-regulated. The C6orf176-TV1 level was correlated to the grade of differentiation (P<0.05) but it was not correlated with gender, age, smoking history, tumor type and TNM stage. The expression of C6orf176-TV1 had a potential value in diagnosis of NSCLC by receiver operator characteristic (ROC) curve. Area under curve (AUC) of ROC curve was 0.708 (95% CI 0.615 to 0. 802). The sensitivity and specificity were 51% and 88%, respectively. For C6orf176-TV2, 39 cases were down-regulated and 18 cases were up-regulated. The C6orf176-TV2 level was correlated with the grade of differentiation (P<0.05) but it was not correlated with gender, age, tumor size, smoking history and TNM stage. C6orf176-TV2 level had value in diagnosis of NSCLC by ROC curve. AUC of ROC curve was 0.64 (95% CI 0.531 to 0.749). The sensitivity and specificity were 49% and 75%, respectively. Of the 57 specimens, 53 cases were simultaneous up or down-regulation of C6orf176-TV1 and C6orf176-TV2. The correlation coefficient was 0.99.
CONCLUSION:The expression of C6orf176-TV1 or C6orf176-TV2 is down-regulated in NSCLC and it is correlated with the grade of differentiation. It may act as a diagnosis indicator for NSCLC patients.
