Y-27632 reduces the MMP2 and MMP9 expression in endothelial cell via inhibition of ROCK signal pathway.
- Author:
Xiaoxiao MAO
1
;
Zhengxiang ZHOU
2
;
Ke XIA
1
;
Qiong DUAN
1
;
Yixia ZHAO
1
;
Dafeng YANG
1
;
Yi XIAO
1
;
Zhenzhen LIU
1
;
Ya WANG
1
;
Tianlun YANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Amides; Cells, Cultured; Down-Regulation; Endothelial Cells; Humans; Intercellular Adhesion Molecule-1; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Pyridines; Signal Transduction; Tumor Necrosis Factor-alpha; Umbilical Veins; Vascular Cell Adhesion Molecule-1; rho-Associated Kinases
- From: Journal of Central South University(Medical Sciences) 2016;41(6):566-570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of ROCK inhibitor Y-27632 on the matrix metalloproteinase 2 and 9 (MMP2 and MMP9) gene expression and activity in tumor necrosis factor α (TNF-α)-treated human umbilical vein endothelial cell (HUVEC).
METHODS:HHUVEC was divided into 3 groups, a control group, a TNF-α group, and a TNF-α plus Y-27632 group. The expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), MMP2 and MMP9 were examined by real-time PCR. The MMP2/9 activity was measured by gelatin zymography.
RESULTS:Compared to the control group, the mRNA expressions of ICAM-1, VCAM-1, MMP2 and MMP9 were increased TNF-α-treated cells, which were suppressed by ROCK inhibitor (P<0.01). The MMP2/9 activity was elevated in TNF-α-treated cells, which was reversed by ROCK inhibitor (P<0.05).
CONCLUSION:ROCK inhibitor can suppress TNF-α-induced inflammation in endothelial cells through down-regulation of MMP2/9.
